We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MCT-1. Sixteen phosphate buffered saline (PBS), boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MCT-1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (,24R)-ergosta-,-diene-,,-triol (1). 1 induced ALP activities of MCT-1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MCT-1 cells by 1, ergosterol (2), ergocalciferol (), cholesta-,,-triol (4), -dehydrocholesterol () and cholecalciferol () were tested. The enzyme activities of MCT-1 cells were increased .0-fold by 10 μM 1 and 2.4-fold by 10 μM 4. However, 2, , and did not induce MCT-1 cell ALP activity at 0.1—10 μM. These results suggested that the OH groups at C- and/or C- of 1 and 4 played an important role in their differentiation-inducing activities on MCT-1 cells. Furthermore, 1 suppressed induction of MCT-1 cell apoptosis by serum starvation.

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It has been shown after mathematical and mechanical investigation that Limit Equilibrium Method (LEM) can be considered as a method to obtain the necessary condition of Slip Line Method (SLM), and that the solution can be obtained under the condition that F_s=1 and ∂F_s/∂θ=O, where F_s is the safety factor and θ is the inclination of the plane on which the safety factor is defined. Next, the generalized LEM (GLEM) is proposed, dealing with the following points : (1) Triangular or quadrangular blocks can be treated; (2) Safety factors are defined also on interblock planes; and () All types of plastic problems, slope stability, bearing capacity, and earth pressure are identically formulated. Two situations regarding treatment of the moment equilibrium condition are discussed. GLEM is applied to well-known problems. The results agree well with those obtained by theoretical methods. GLEM can be used to obtain the distribution of earth pressure or bearing capacity. GLEM, Iike ordinary LEM, is very effective for the practical problems, but GLEM is more theoretical and it can result in more accurate solutions.

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ラットに

3H

-CU-

83

(S)を25μg/kgで静脈内あるいは経口投与し，血液中濃度および尿糞中排泄を検討した．
静脈内投与後の血液中濃度推移は投与後

5

分より上昇し，投与後45分に25.74ng eq./mlのC_maxを示し，それ以後t_1/2

3

.05時間とt_1/2 33.09時間の二相で減少した．投与後72時間までのAUCは135.42ng eq.·hr/mlであった．
経口投与では，投与後

3

時間でC_max 4.10ng eq./mlに達し，以後t_1/2α 4.46時間とt_1/2β 26.

83

時間の二相で減少した．投与後72時間までのAUCは48.62ng eq.·hr/mlであった．
静脈内投与と経口投与のいずれの場合も，尿および糞中への放射能の排泄は，投与後48時間でほぼ終了した．静脈内投与では，投与後72時間までに投与量の30.52%が尿中に，60.42%が糞中に排泄された．経口投与では，同じく72時間までに40.34%が尿中に，69.24%が糞中に排泄された．

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Metabolism of body fluids and electrolytes which related not only to aldosterone alone but to other corticosteroids, for example, glucocorticoids had mineral action. And the simultaneous administration of aldosterone and corticosterone consistently resulted in a greater potassium diuresis than did the aldosterone alone.
Therefore, body fluids and electrolytes are aummation of many corticosteroids which had biological action, and can't know by the determination of simple corticosteroids.
The author report on the systemic determination of aldosterone, Comp. , Comp. , Comp. , and the newly separated contaminants.
Method :
The 24 hours urine specimen is pooled at °C and acidified to pH 1. with conc. HCL, then standing 24 hours at 15°C.
Urine was extracted by chloroform and dried. Extract was treated with Florisil column chromatographies and 4 consecutered and different paper chromatographies with Chloroform-Formamide System, Bush-C System, System, and Bush- System, to separated corticosteroid partitions.
Results :
1. Recoveries of mg/100 ml H₂O standard samples after times continued extraction by 0.1 volume chloroform, for Comp. , Comp. , and DOC is 95%, and Comp. is %.
2. Additional chloroform re-extraction, Comp. , Comp. , and DOG do not appear in the th chloroform extraction, but Comp. appear in the amount of 60μg in th chloroform and 9th extraction is nothing, thus this determination necessitate at least consecutered extractions.
. Comp. , Comp. , and Comp. appear in 25% methanol in chloroform fraction by the use of the Florisil column chromatography is made, and recovery is about 100% in condition of 60 drops/minutes.
4. Separate Comp. III, Comp. V, Comp. VI, and Comp. VII from aldosterone partition in Neher-Wettstein's method.
. New contaminants were discover from Comp. and Comp. partition after Bush-C System paper chromatography, called Comp. -, Comp. ; and, other conatminants named M₁- Z₁-, HC₁-, and in Chloroform-Formamide and Bush-C System paper chromatography.
. Examination of the purities of Comp. , Comp. , Comp. , and aldosterone partitions is made use of the ultra violet absorption spectrum, and necessitates 4 (C-→Bush-C→→Bush-), or (C-→→Bush-), paper chromatographies to satisfactorily separate these compounds.
. The H₂SO₄ chromogen spectrum of these partition correspond with the standard compounds.

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The possibility of attenuation of microseismic waves due to the presence of an upward folding of the ocean bottom into the liquid is discussed in this paper. The upward folding of the ocean bottom has been idealized by means of an irregularity in the form of a rectangle intruding into the liquid and the evaluation of the displacement component at any point of the solid medium has been carried out in two stages. First, the change in the incident mode of the surface wave due to the presence of the rectangular irregularity has been determined, and second, the subsequent change of the resulting displacement components due to the termination of the liquid layer and the step change in elevation of the solid medium at the continental margin has been discovered. Finally, numerical calculation has been made to determine the attenuation of the microseismic waves of different periods due to the presence of the rectangular irregularity at the ocean bottom.

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A method for analyzing the uncertainties involving the use of empirical relationships in design is developed. It is applied to evaluate the reliability of the relationship s_u(mobilized)=(measured), whereby the pertinent uncertainties are analyzed using extensive laboratory and field observed data. On the average, the relationship s_u(mobilized)= (measured) is unbiased and the error implicit in the relationship is about 15%. When cone values are used to infer s'_p, additional uncertainties on the mobilized undrained strength will incur resulting mainly from the calibration uncertainty between σ'_p and the cone values. Alternatively, vane tests may be performed to infer the mobilized strength using Bjerrum correction factor. Results show that uncertainty level associated with using the vane tests is smaller than that using the cone values, provided that similar scatter is observed between measured vane and cone values at a given site. However, prediction of the mobilized strength is even more accurate if direct measurements can be made on the preconsolidation pressure. The proposed probabilistic method also assesses the benefit of additional tests besides comparing the accuracy of using various soil parameters for predicting mobilized undrained strength. Such information is essential for planning cost-effective site characterization program.

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The modulns of longitudinal elasticity and their temperature dependence in commercial aluminum cast alloys were measured in the temperature range from room temperature (20°C) to 250°C by the resonant frequency method. The results obtained are as follows:
The modulus of longitudinal elasticity tended to decrease weakly and linearly (the tangent of its decrease, m = (-2.4--.) × 10²kg/cm²/°C) with the rise of temperature. Within the range of the experiments, aluminum cast alloys were divided into groups, the lst group materials having large moduli of elasticity at room temperature (i. ., = (.15-.25) × 10⁵kg/cm²) were 2A-T, A-, 4A-, 4A-T4, 4-, 4-T4, A-, A-T4, etc., the 2nd group materials having intermediate moduli (i. ., = (.85-.98) × 10⁵kg/cm²) were 4C-, 4C-T, 4C-T, 4D-, 4D-T, A-, etc. and the rd group materials showing considerably smaller moduli (i. ., = (.58-.65) × 10⁵kg/cm²) were 1A-, 1A-T, 2A-, etc. However, there were some differences between the moduli in this experiments and the moduli in the other data books, and these were considered due to the difference of the measurement principles and of hypotheses or assumptions involved in these principles, so it is suggested that the moduli of elasticity should be reported with indication of the measuring method.

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The Tsukuba Challenge is a competition, in which autonomous mobile robots run on a route set on a public road under a real environment. Their task includes not only simple running but also finding multiple specific persons at the same time. This study proposes a method that would realize person searching. While many person-searching algorithms use a laser sensor and a camera in combination, our method only uses an omnidirectional camera. The search target is detected using a convolutional neural network (CNN) that performs a classification of the search target. Training a CNN requires a great amount of data for which pseudo images created by composition are used. Our method is implemented in an autonomous mobile robot, and its performance has been verified in the Tsukuba Challenge 2017.

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The numerical values of the intervals betrween optieal levels are competed for the configurations *⁹4s, s, s and s of Cu⁺, according to the general expression of energy-levels derived in the previous paper The self-consistent field radial functions computed by Hartree adn Hartree are used for1s, 2s, 2p, s, p and d. Those of 4s, s, .s and are ealenlated from Hartree Hartree's core-functions by the numerical integrations. The calculated results are shown in Table I.The agreement with experiment is satisfactory

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The acetone extracts from three samples of the hepatopancreas of the poisonous scallops obtained on the Okhotsk Coast of Hokkaido Island were fractionated into two parts, hexane soluble fraction (fraction H) and 85% aqueous ethanol soluble fraction (fraction ) by partition to two layers. The majortoxic components in the mouse assay of “diarrheic shellfish toxin” by intra-peritoneal injection were found to be free unsaturated fatty acids showed the following toxicity in MU per g, 18:1 n-9 35, 18:2 n- , 18: n- 167, 18:4 n- , 20: n- 167, and : n- , respectively. Toxicity of the fraction Hin MUper g was much lower than that of the fraction . However, the toxicity of the fraction H per 1 g of the hepatopancreas was about twice that of the fraction , since the fraction Hwas much more abundant than the fraction in the hepatopancreas. The method for the assay of the diarrhetic shellfish toxin must be reexamined by considering the toxic effect of the free unsaturated fatty acids.

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The pseudo-static analysis has been used in engineering practice to simplify the computation of the upper limit of a slope under seismic load. In this paper, the upper bound technique of limit analysis of perfect plasticity is used to determine the bearing capacity of a foundation near a down-hill slope. In this analysis (Chen et al., 1969; Chen, 1975), a logarithmic spiral rapture (Abdul-Baki et al., 1970; Sawada et al., 1984) is assumed to start at an edge of the loaded area far from the slope. A landslide is assumed to be of rigid body type with the inertia force acted at its center of gravity. The collapse state is reached when the rate of kinematic energy of the landslide together with the load is equal to the rate of dissipation of internal energy along the sliding surface. This energy balance leads to the equation of ultimate load with several parameters. The optimized numerical rcsults with respect to these parameters are compared with those by Kotter's method (Yamaguchi, 1959) and those obtained by the slice method.

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