Immunosensor Using Surface Plasmon Resonance for C-Reactive Protein Detection

抄録

An immunosensor for human C-reactive protein (CRP) detection was developed by combining a portable surface plasmon resonance (SPR) machine with an anti-human C-reactive protein monoclonal antibody (AbCRP)-immobilized gold surface of the microfluidic SPR sensor chip (MSSC). The gold surface of MSSC was chemically modified using self-assembly monolayer technique with 4,4′-dithiodibutyric acid. Subsequently, AbCRP was immobilized on the chemically-activated gold surface of the MSSC by chemical adsorption and CRP determinations were performed using immunoreaction. The detection limit for CRP concentration by this developed SPR method was 1.0 µg/ml.