Suppression of SOS-Inducing Activity of Chemical Mutagens by Yakuchinone A from Alpinia oxyphylla in the Salmonella typhimurium TA1535/pSK1002 umu Test

抄録

The methanol extract from Alpinia oxyphylla showed a suppressive effect on umu gene expression of SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide). The methanol extract from A. oxyphylla was successively re-extracted with CH₂Cl₂, EtOAc, BuOH, and water. A dichloromethane fraction showed a suppressive effect. The suppressive compound in the dichloromethane fraction was isolated by SiO₂ column chromatography and identified as yakuchinone A(1) by GC, GC/MS, ¹H and ¹³C NMR spectroscopy. Compound 1 suppressed the SOS-inducing activity of furylfuramide in the umu test. Compound 1 suppressed 51.7% of the SOS-inducing activity at a concentration of 0.32μM/mL. The ID₅₀ value (50% inhibition dose) of compound 1 was 0.30μM/mL. This compound was assayed with an other mutagen, 3-amino-14-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which requires a liver metabolizing enzyme, activated Trp-P-1.