抄録
A novel series of fucolipids, provisionally named CPS and CHS in the previous study, was obtained from whole tissues of the marine annelid, Pseudopotamilla occelata. The structures of these fucolipids were determined by compositional analysis, methylation analysis, gasliquid chromatography, gas chromatograph-mass spectrometry, proton nuclear magnetic resonance spectroscopy, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The structures were shown to be Xylβ1-4Fucα1-3GlcNAcβ1-3Galβ1-4Glcβ1-1Cer (CPS1) and Gal2Meα1-3Fucα1-3GlcNAcβ1-3Galβ1-4Glcβ1-1Cer (CPS2) for CPS, and Xylβ1-4 (Gal2Meα1-3) Fucα1-3GlcNAcβ1-3Galβ1-4Glcβ1-1Cer for CHS, respectively. They were structurally related to the previously described ceramide trisaccharide (GlcNAcβ1-3Galβ1-4Glcβ1-1Cer, Amino-CTH) except that a fucose-containing di- and/or trisaccharides were linked to the N-acetylglucosamine residue of the latter lipid. The predominant fatty acids were monoenoic acids, C20:1- and C22:1 acids amounting to about 80% of the total acids, and octadeca-4-sphingenine was the sole sphingoid base. The fatty acid and sphingoid base compositions showed nearly the same distribution in these fucolipids.
