抄録
Reactive Oxygen Spiecies (ROS) such as superoxide anion radical (·O2-) act as signals for the activation of stress-response and defense pathways. However, excess ROS generated by perturbing ·O2- homeostasis stimulated many environmental stress, including intense light, drought, temperature stress, herbicides, induce high radical toxicity. Consequently, quantitative analysis of ·O2- is a subject of intense research, since most of ROS are derived from ·O2-. Iron meso-tetrakis(3-thienyl)porphyrin complexes were electropolymerized onto a Au wire electrode. The modified Au electrode were applied to ·O2- sensor to detect catalytic oxidation current of ·O2- which was generated as an intermediate during the oxidation of xanthine by catalystic XOD. It was revealed that the sensor was quantitative to measure ·O2-. The modified Au electrode were applied to measure oxidation current of ·O2- in mung beans under environmental stress condition. Plants were grown in atmosphere, 25°C and in black darkness. The other plants were exposed to oxygen excess. The oxidation current of ·O2- were increased plants were grown by high-oxygen environment compared to plants were grown at atmosphere. This experiment was indicated that environmental stress such as hyperoxia induced excess ·O2- and Au wire sensor using iron porphyrin complexes is capable of ·O2- detection in plants under environmental stresses.
