含トコフェロール濃縮物の利用に関する研究 (第1報)

薄層クロマト法による構成成分の分離と還元性物質の二, 三の性質について

抄録

It is generally accepted that the deodorized condensates, obtainedin the refining process of vegetableoils, contain 1 to 10% tocopherols.
The condensates are good raw materials for the preparation of α-tocopherol or tocopherol concentrates.
The concentrate containing 42.6% tocopherols as α-type was prepared by means of esterification and distillation of the soybean oil condensate.
Thin-layer chromatography was applied to analyze the concentrate components prior to studies on utilization of the concentrate, and the following results were obtained ;
1) For the separative analysis of these components, nonpolar and polar developing solvent systems were at least necessary. Hydrocarbons, squalene and five other unknown components were separated eachother in nonpolar sopolar solvent system such as petroleum benzene. On the contrary, α, γand δ-tocopherol, higher alcohols and sterols were separated to give each spotsin polar solvent system such as n-hexane : ether (7 : 3 vol/vol).
2) These spots were possible to detect by means of UV illuminating and such solution spraying as iodine-benzene, 50% sulfuric acid or Emmerie-Engel reagent.
3) Fractionation of the components was possible to separate into six fractions on thin-layer chromatographic plate. These fractions were ; least polar fraction (I) such as hydrocarbons and squalene, intermediate polar fraction (II) such as tocopherols, most polar fraction (III) such as alcohols and sterols, (I) + (II), (I) + (III), and (II) + (III), respectively.
4) With the exception of the above described components, other two components were recognized to have reducing power by means of coloration with Emmerie-Engel reagent and from consumption of ceric ammonium sulfate. Furthermore, inspection on IR and UV spectra suggested that they were β, γandδ-tocopherol derivatives substituted with nonpolar large radicals for the hydrogen atom at 5 or 7 positionin benzene ringof tocol.