トリグリセリドのGC-MS分析(第3報)

構成脂肪酸分布と特定フラグメントイオン強度比の関係

抄録

GC-MS analysis permitted the determination of the fatty acid composition of triglycerides with the same carbon number. Then the most useful specific fragment ions are RCO⁺, and [RCO+128]⁺. Their intensities, however, are not always proportional to the amount of corresponding fatty acids present. Thirty diacid triglyceride (triglyceride molecules containing two different fatty acids) were synthesized, and investigated about the disproportionality. These samples comprise every possible diacid triglycerides of C₈, C₁₀, C₁₂, C₁₄, C₁₆, and C₁₈ saturated straight chain fatty acids.
The intensity ratios of RCO⁺ were compared with the actual content ratio between two constitutive fatty acids. The intensity of RCO⁺ of longer chain fatty acid was always smaller than that of the shorter ones. This tendency was more significant increasing with the difference of carbon number between two constitutive fatty acids. The apparent intensity of [RCO+128]⁺ was influenced by [RCO+128+14n]⁺(n≥1).