The neutral lipid containing γ-1inolenic acid was separated by supercritical fluid chromatography(SFC). By adding hexane or methanol to supercritical carbon dioxide, the separation was more rapid and effective. The fatty acid composition of each fractionated peak was studied. In the initial elution part of the fraction of triglyceride, γ-1inolenic acid was present by 2 to 5 times as much as that in the original neutral lipid.
