1992 年 41 巻 12 号 p. 1171-1179
A method for determining phospholipid composition was developed by ten laboratories working in collaboration. Commercially available soybean lecithin and egg yolk lecithin were used. Phospholipids were separated by high performance liquid chromatography using with a stainless steel column (φ 3 mm × 150250 mm) packed with silica gel (particle size 5 μ m) and UV detector (206 nm).
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidic acid in soybean lecithin could be clearly separated with the mobile phase of hexane/isopropyl alcohol/0.2 M acetate buffer (pH 4.2) =8/8/1 (vol/vol).
Sphingomyelin and lysophosphatidylcholine were also separated from the above four major phospholipids. The detector responce of these phospholipids for lipid phosphorus content showed good linearity.
Repeatability and reproducibility shown by statistical analysis of the results indicated the present method to provide highly accurate results for major phospholipids of commercial lecithin.