Two coumarin derivatives were isolated from hassaku (Citrus hassaku HORT.), zabon (Citrus gradis OSBECK.) and amanatsu (Citrus natsudaidai) peeling. Their structures were determined by means of MS, 1H-NMR and 13C-NMR spectroscopy.
8- (2, 3-dihydroxy-3-methylbutyl) -7-methoxycoumarin (1) and 7- [(6, 7-dihydroxy-3, 7-dimethy1-2 E-octenyl) oxy] coumarin (2) were found.
Compound (1) was intravenously injected into SHR-SP rats (1 mg/100 g body weight) with subsequent rise in blood pressure. Compound (2) was not tested owing to its poor solubility in water.
