抄録
A comparative study was made on fatty acid profiles of lipids in various tissues and feces from rats fed for 28 d one of the following diets 20 % in fat content : soybean oil mixed either with trans- or cis-dococenoic acid (TDO : t-22 : 1 18.1 %, CDO : c-22 : 1 18.2 %) blended rapeseed oil (RSO : c-22 : 1 17.9%) and soybean oil (as control).
1) The proportion of t-22 : 1 was markedly low (0.10.4%) in all tissues examined in rats fed TDO, but slightly higher in adipose tissue (0.6%) and adrenal (0.5%). The proportion in fecal lipids was extremely high (51.3%). The same has been noted for c-22 : 1, but the proportion of c-22 : 1 in each tissue of rats fed CDO and RSO was slightly higher (0.57.2%) than that (0.12.6%) of t-22 : 1.
2) Virtually no dietary t-22 : 1 was incorporated into liver phospholipids. Incorporation into liver subcellular granules was only slight, as also observed for c-22 : 1. The proportion of c-22 : 1 was lower in liver subcellular granules, but higher in the cytosol fraction, compared to that in whole liver.
3) In serum lipids, the proportion of t- or c-22 : 1 in HDL phospholipids was slightly higher than that in LDL phospholipids.
4) Dietary long-chain trans-monoenoic acids present in hardened fish oil, would thus appear to be preferentially excreted into feces and incorporated into various tissues in very small amounts, particularly in the case of liver subcelullar granules and membranes, even when fat contains trans acids at considerably high levels. The incorporation of long chain cis-monoenoic acids into various tissues was slightly higher than that of the corresponding trans acids.
