日本食品微生物学会雑誌
Online ISSN : 1882-5982
Print ISSN : 1340-8267
原著
クオリバックスTM Q7システムを用いた食品からの腸管出血性大腸菌の迅速検出法の検討
森 哲也畑 ますみ渡辺 晃正岩出 義人和田 真太郎難波 豊彦遠山 一郎大熊 周平上橋 健三小沼 博隆伊藤 武
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31 巻 (2014) 4 号 p. 194-203

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The purpose of this study was to evaluate the QUALIBAXTM Q7 System (BAX system) for detecting non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups (O26, O103, O111, O121, O145) and STEC O157. The BAX system real-time PCR assay STEC suite was evaluated, including a screening assay for the stx and two panel assays to identify the target serogroups. For STEC O157, BAX system real-time PCR assay E. coli O157 : H7 was used. The BAX system assay was found to detect STEC O26, O103, O111, O121, O145 and O157 in pure culture. Each assay was 100% inclusive for the STEC strains used. And no cross-reactivity was observed. Sensitivity of a screening assay for the stx was ≧1.8×103 CFU/ml in cultures of ground beef or lettuce. E. coli O157 : H7 was detected at a level of ≧1.8×104 CFU/ml. On a collaborative study, the performance of the BAX system assay was compared with that of a culture method for detection of STEC O26, O111 and O157 in ground beef and lettuce. Sensitivity of the BAX system method was better than a culture method. These results suggest that the BAX system can be used as a rapid method for detecting STEC in food samples.

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