関東東山病害虫研究会報
Online ISSN : 1884-2879
Print ISSN : 1347-1899
ISSN-L : 1347-1899
病害の部
我が国で発生する8種トスポウイルスを検出できるユニバーサルプライマー
奥田 充
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ジャーナル フリー

2017 年 2017 巻 64 号 p. 68-72

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To develop an assay using reverse transcription polymerase chain reaction (RT-PCR) to detect multiple species of viruses in the Tospovirus genus, six primer pairs were chosen from those previously reported and tested to determine if RT-PCR with these primers could detect 8 species occurring in Japan. Consequently, two primer sets, gL2740/gL3920 or t2740/t3920c could detect all species occurring in Japan. The nucleotide residues of t2740 and t3920c were modified to correspond with each of the target sequences of 19 species reported outside Japan, except Groundnut chlorotic fan-spot virus. RT-PCR with the modified primer pair resulted in detecting the 8 species occurring in Japan, and was expected to be available for multiple species in the Tospovirus genus.

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© 2017 関東東山病害虫研究会
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