メロンえそ斑点病発病土壌から効率的に病原ウイルスを検出するための条件の検討

抄録

For detection of melon necrotic spot virus (MNSV) from infected soils, enzyme-linked immunosorbent assay (DAS-ELISA) has been applied to root samples of melon seedlings, pot-cultivated in the soil for three weeks in a growth chamber at 25°C in 16-h light / 8-h dark conditions. In order to increase the detection accuracy, it was shown that the incubation of sample seedlings should be carried out at 18°C or 20°C. Moreover, MNSV was detected by DAS-ELISA from all samples incubated for two weeks at changing temperature conditions (25°C or 30°C in light and 15°C in dark) showing that the test period was shortened by one week compared to plants incubated at a constant temperature condition (25°C in light and dark).