Seventy-eight plant samples of Chinese peony (Paeonia lactiflora Pall.) in Japan were collected from five prefectures to survey viral pathogens. By next-generation sequencing, cycas necrotic stunt virus (CNSV) and tobacco rattle virus (TRV) were detected. As a result of performing RT-PCR for each individual sample, CNSV was detected from a plant exhibiting mottle, whereas TRV was detected from three plants exhibiting ringspots, line patterns or yellow blotching on their leaves. This is the first report of detection of CNSV by RT-PCR from Chinese peony in Japan.
