Two subpopulations of young sandeel Ammodytes personatus were found off Tottori Pref. by examining a combination of the vertebral count and gill raker number. These two subpopulations reveal differences also in size and stomach contents. Coexistance of two subpopulations in this area seems to be closely related to the oceanographical conditions: eastward Tsushima Warm Current encountering southward Japan Sea Central Water in spring.