抄録
The triglyceride (TG) and fatty acid compositions of rock crab Cancer irroratus, lipid were investigated by using reversed-phase high performance liquid chromatography (HPLC) of TG in combination with gas liquid chromatography (GLC) of fatty acids. After crab meal neutral lipid was separated into an acetone-soluble fraction (mainly neutral lipid, SNL) and a precipitate fraction (PF), each fraction was first examined by HPLC. TG in SNL, which had a high con-tent of polyunsaturated fatty acids such as 20:5 and 22:6, consisted of components with partition numbers (PN) of 36 to 52; however no good separation among these was observed on the chro-matogram.
On the other hand, TG in PF readily separated into twelve peaks of PN 44 to 56. Weight percentages of the main peaks of PN 48, 50, and 52 were 26, 26, and 21%, respectively. As a result of GLC analysis, the fraction with PN of 44 was shown to have a high content of polyenoic acids, concentrated five times higher in mole % than that of total PF. Fractions of all PN had more than 20mole % of C16:0. It is suggested that one or more molecular species type in PF might be dominated by C16:0. In this experiment, two or three peaks having the same PN were resolved by using reversed-phase HPLC. In these components with the same PN, the early-eluting peaks had a high amount of C18:1 or other monoenes, while the later-eluting peaks had much more C16:0.
