抄録
A neutralβ-N-acetylglucosaminidase was purlfied from carp blood by a combination of ammonium sulfate fractionation, Cellulofine GCL-2000 gel filtration, DEAE-cellulose chromato-graphy, hydroxyapatite column chromatography, Mono Q column chromatography, affinity chromatography on ρ-aminophenyl-2-acetamido-2-deoxy-β-D-glucosamine coupled with CNBr-activated Sepharose 4B and chromatofocusing on Mono P column. The specific activity of the enzyme was increased about 1425-fold in 2.4% yield, and the final preparation proved to be homo-geneous on polyacrylamide disc gel electrophoresis.
