抄録
Free biotin in blood was microbiologically assayed according to Skeggs・Wright. Total content of biotin was estimated after hydrolysis with 3 N H_2SO_4 for 30 minutes at 120℃. Biotin content in normal rabbit serum was 19.5 mμg/ml as total, and about 98.4% of the total was free biotin. After second sensitization with typhoid-paratyphoid vaccine, biotin content in serum was decreased remarkably and reached about 77% of normal value at two hours later, since inosine content was increased at the time as previously reported. When a large quantity of inosine (5 mg/kg) was preinjected between first and second sensitization with typhoid-paratyphoid vaccine, biotin content in serum did not vary with the second sensitization. These results suggested that the two compounds, biotin and inosine, were corelated in metabolic control system of rabbit liver.
