Type 1 diabetes (T1D) is an autoimmune disease in which blood glucose level is uncontrollable due to the destruction of pancreatic islet β-cells by auto-reactive immune cells. If immune suppression specific for β-cell-derived antigens, namely the immune tolerance could be induced, it would be possible to stop proceeding with destruction of β-cells at the early stage, and the dependence of patients’ insulin would be reduced. Moreover, if transplanted pancreatic islet grafts could be kept without continuous administration of immunosuppressants, patients would spend insulin-free lifetime. T1D arises from the breakdown of immune tolerance towards pancreatic β-cells that may result from defects in natural killer T (NKT) cells and regulatory T cells (Tregs) function. Given the similarities in the pathogenesis of disease between humans and NOD mice, we hypothesize that the activation of NKT cells may modulate the onset and severity of T1D. To date, the most powerful activator of NKT cells described is a synthetic α-galactosylcermide (α-GalCer). NKT cells activated by αGC rapidly produce large quantities of immunomodulatory cytokines, including IL-2, IL-4, IL-10 and IFN-γ. Of note, IL-2 and IL-10 are key cytokines for both the survival and expansion of Tregs and for promoting the generation of tolerogenic antigen-presenting cells (APCs). Thus, NKT cells have multiple functions by which they may induce the immune tolerance to self-reactive lymphoid cells. The contrasting effects of aqueous vs. liposomal delivery of α-GalCer have been attributed to differential cell targeting. Aqueous forms of α-GalCer are presented primarily by professional APCs, dendritic cells (DCs) whereas liposomal α-GalCer delivery results in presentation by B cells and subsequent IL-10 secretion by both B cells and NKT cells, production of tolerogenic DCs and generation of Foxp3
+Tregs. We show the mode of action and the therapeutic potential of liposomal α-GalCer to protect exacerbation of T1D.
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