Feulgen's procedure is one of the useful observation techniques on the mitotic divisions in plants. Chromosomes and nuclei are effectively stained by Feulgen's procedure. Thus the present procedure has been favourably used as an excellent technique in cytological studies. However, it was clarified that the Feulgen method should be partially improved from the viewpoint of science education. The Feulgen method improved by the present author is as follows: ① collection of fresh root tips, ② washing, ③ fixation with acetic alchol (1:3) at 10℃ for 4-24 hr, ④ rinsing with ethanol series, 75%, 65%, 50%, 30% and 15%, for 15 min respectively, ⑤ hydrolysis with IN HCl at 60℃ for 8 min, ⑥ washing, ⑦ staining with Schiff’s reagent at room temperature for 1-2 hr, or at 7-10℃ for over 12 hr, ⑧ washing shortly, and ⑨ squashing with 0.2% acetic orcein.
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