The purpose of this study was to examine the effect of different chromatic light environments on human movement. Sixteen members(8 males and 8 females)participated in this study. The subjects were tested for the center of gravity sway, muscular strength, and operation accuracy under both Daylight LED and Red light LED chromatic light environment conditions. The electromyogram of both males and females were also analyzed under both conditions. The differences in the ability of movement of males and females in both the chromatic light environments were explored. The males operation accuracy was higher under Red light LED chromatic light environment conditions than under Daylight LED. This has a positive effect on darts in Red light LED chromatic light environment. The results of this study showed that males are more likely affected by Red light LED chromatic light environment than females.