Objective: This review aimed to clarify the association of rotating shift work, including night shifts, with the menstrual cycle through meta-analysis of previous studies. Methods: Four electronic database searches and hand searches were conducted using search terms such as rotating shift work and menstruation for Japanese and English literature published up to February 2024. Inclusion criteria were as follows: The subjects were menstruating women, exposure was rotating shift work including night shifts, the comparison group was non-rotating shift work without night shifts, outcome was irregular menstrual cycles, long or short menstrual cycles, and menstrual duration, and the study design was observational. The DerSimonian-Laird method was used in the meta-analysis. Results: Twelve articles were included in this review. In the rotating shift work group, five of six articles indicated an increased risk of irregular menstrual cycles, four of six indicated an increased risk of long menstrual cycles, and three of seven indicated an increased risk of short menstrual cycles. The meta-analysis showed that the rotating shift work group had a significantly higher risk of irregular menstrual cycles [odds ratio (95% confidence interval) = 1.51 (1.32–1.72), integrated from five articles (n=17822)] and long menstrual periods [1.70 (1.00–2.87), integrated from five articles (n=10225)] than the comparison group. In contrast, no association was found between rotating shift work and short menstrual periods or abnormal menstrual durations in the meta-analyses. Conclusion: Women who work rotating shifts, including night shifts, need to understand the risks of irregular and long menstrual cycles so that abnormalities can be detected early.
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