Poor vegetation due to acidic soil have occurred on many cut slopes of the expressways, although acidic soil had not been recognized in Shikoku. This study clarifies that the occurrence of acidic soils on the cut slope of the Shikoku Expressway are concentrated along the Median Tectonic Line. The acidic soils on the cut slopes of the expressway along the Median Tectonic Line is caused by oxidation of cubic pyrites which was formed by hydrothermal alteration due to the Miocene volcanism. The acidic soils became apparent in the unweathered bedrock which was exposed on the large-scale cut slope where pyrites have not been oxidized from near the ground surface. Hydrothermal alteration due to Miocene volcanic activity is the main cause of acid soils along the Median Tectonic Line.