Liver glycogen and several blood constituents were determined in fasted and non-fasted roosters injected with the same dosage of estrogen (Euvestin sol TAKEDA). The 72-hour-fasting was significantly effective in lowering the plasma glucose and liver glycogen, but was not effective in the NaCl-Ac turbidity and plasma calcium values.
Additions of Heparin-Na to the serum of estrogenized roosters caused a striking increase of the NaCl-Ac turbidity value. This phenomenon suggests that the serum lipoprotein forms a heprin-lipoprotein complex, not soluble in the NaCl-Ac solution. The faint NaCl-Ac turbidity observed in the heparinized plasma of untreated roosters, also may be interpreted as the result of the same phenomenon.