Age changes of the periodontal ligament of the molars were studied by morphometry using senescence accelerated mouse. These mice were from 2 to 16 months of age, and of both the resistant strain (SAMR1/Iw) and prone strains (SAMP2/Iw and SAMP8//Iw). The width and the cell number of the periodontal ligament at four parts of the maxillary first molar (M1), namely the mesial side of the mesial root (M), distal side of the distal root (D), furcation of Ml (F), and apex of the mesial root (A), were examined under light microscopy using IBAS-2000. Then, the numbers of epithelial rest of Malassez and of cementicles were counted in the periodontal ligament surrounding the mesial root and the distal root of the maxillary M1. Mean scores of the histological parameter were calculated, and the strain and age differences were statistically analyzed by analysis of variance.
The width and the cell number of the periodontal ligament were different according to the parts. No strain difference was seen in the width of the periodontal ligament at all four parts. At (M) and (A), the width of periodontal ligament did not change with age, but it decreased at (D). At (D), it was significantly different for 2 and 16 months of age in each strain. At (M) and (F), the cell number in the periodontal ligament did not change, but it tended to increase at (D). At (A), the cell number was slightly higher at 2 months of age in all strains, but thereafter they showed the same value throughout the experimental period. The epithelial rests of Malassez were often seen at the neck and the furcational area of the periodontal ligament. The numbers of epithelial rests of Malassez did not show any strain differences, and they were high in the young group and decreased with age. Cementicles were often observed in the apical periodontal ligament of the root. The numbers of cementicles also showed no strain differences, and increased with age.
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