The genetic relationship among 15 lettuce cultivars (Lactuca sativa L.) was examined by RAPD analysis using 12 dodecamer primers singly or combining two primers in a single PCR. The cultivars examined were classified into three groups based on the numerous polymorphisms of DNA fingerprints. The main group consisted of thirteen cultivars with crisphead lettuces having compact leaves form tight head (6 cultivars), such as 'TE242', 'Shizuka', 'Shisuko', 'King Shisuko', 'Sodegaura', 'V lettuce', a cultivar looseleaf lettuces with a bunch of green or red leaves (5 cultivars), such as 'Redfalder', 'Banchu Redfire', 'Chimanchu', 'Dancing', 'Red Wave', cultivar cos lettuces having long upright leaves (1 cultivar), such as 'Romaine lettuce' and stem lettuces like big asparagus shape with thin leaves (1 cultivar), such as 'Stem lettuce'. The second group included 1 cultivar of cos lettuce such as 'Cos lettuce' and the third group included a stem lettuce cultivar, 'Cairn'. The largest genetic distance was the relation with 'Cos lettuce' and 'Cairn', while the smallest genetic distance was found among the crisphead lettuces, such as 'TE242', 'Shizuka' and 'Shisuko', and looseleaf lettuces, such as 'Redfalder', and 'Banchu Redfire'.
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