We investigated the effect of the combined treatment with hyperthermia and epinephrine of human oral carcinoma (KB cells) transplanted subcutaneously in the leg of BALB/cA nude mice. Upon obtaining tumors of about 8 mm diameter, we performed the following treatments. Hyperthermia was carried out in a water bath at 39, 41 and 43°C for 30 minutes. Epinephrine was injected intraperitoneally or intratumorally. Single or combined treatments were performed on days 0, 3 and 6, and tumor size was measured. The relative mean tumor weight (RW) compared with day 0 was calculated. The relationship of combined treatment and individual anti-tumor effects were compared by the ratio of the relative mean tumor weights for the treatment and control groups. The potentiation ratio of the effect of epinephrine with hyperthermia was calculated for each dose and concentration.
We found that intratumoral, intraperitoneal injection of epinephrine alone, and combined treatment with hyperthermia and epinephrine injected intraperitoneally had no beneficial effects. Combined treatment with hyperthermia and epinephrine injected intratumorally seemed to have an inhibitory effect on the growth of the KB cells. The sensitizing effect depended on the concentration of epinephrine.
We concluded that the combination of hyperthermia and intratumoral injection of epinephrine is an effective treatment. Shika Igaku (J Osaka Odontol Soc) 1996 Sept; 59 (3): 227-236.

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We analyzed the results of radiation therapy and evaluated its effectiveness as a treatment method. Between 1967 and 1980, a total of 200 patients with squamous cell carcinomas of the tongue (T2N0M0, stage II by UICC) were treated with interstitial brachytherapy alone or in combination with external irradiation (combined therapy) at the Department of Radiology, Osaka University Medical School.
We found that there was no significant difference between interstitial brachytherapy alone and combined therapy with regard to local control rates. With interstitial brachytherapy alone, local control rates for the Ir-192 remote afterloading method were greater than those for Ra-226 needles. The incidence of neck metastases out of the irradiated field was higher with combined therapy than with interstitial brachytherapy alone.
We found that irradiation treatment with thin Ir-192 wire using the afterloading method produced excellent results for patients with carcinomas of the tongue (T2N0 cases). Shika lgaku (J Osaka Odontol Soc) 1996 Sept; 59(3): 237-243.

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Using microdialysis, we evaluated the effect of noxious stimulation on the prefrontal cortex during anesthesia, and the role of AMPA/kainate receptors. We also examined the effects of preemptive analgesia (PA) on the dopaminergic neuron system in the prefrontal cortex. Electrical stimulation of the dorsum pedis decreased dopamine (DA) by 20% (p＜0.05). Electrical stimulation after addition of CNQX, an AMPA/kainate receptor antagonist, to the dialyzing solution further decreased DA.
These findings suggest that noxious stimulation during general anesthesia, unlike during arousal, decreases DA in the prefrontal cortex, and that AMPA/kainate receptors inhibit this decrease. In addition, noxious stimulation during anesthesia decreases DA in the prefrontal cortex, although epidural anesthesia inhibited this decrease. Shika lgaku (J Osaka Odontol Soc) 1996 Sept; 59(3): 244--25.

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We conducted a clinical and histopathological study of a patient taking Cyclosporin to evaluate both the pathogenesis and treatment of gingival hyperplasia induced by this madication. We evaluated the plaque control record (PCR), probing depth (PD), gingival index (GI), gingival bleeding index (GBI), gingival overgrowth index (GOI) and tooth mobility (TM). Evaluations were made during the patient's initial examination, after initial preparation, after periodontal surgery and during the maintenance recall phase. PCR, GI and GBI improved markedly follwing initial preparation, although no changes were detected in either GOI or TM. GOI and TM subsequently improved with resectioning of the hyperplastic gingiva. However, gingival hyperplasia recurred during the maintenance recall phase.
These observations suggest that the primary cause of Cyclosporin-induced gingival hyperplasia may be the pharmacological activity of the drug. Regional factors such as plaque may only play a secondary role, exacerbating the hyperplasia. Histopathological study of the gingival specimens obtained during surgery revealed chronic inflammation characterized by parakeratinization of the gingival epithelium, thickening of the prickle-cell layer, hyperplasia of the collagen fibers, and infiltration of both plasma cells and lymphocytes into the subepithelial connective tissue.
These results indicate the importance of not only plaque control, scaling and root planing, but also of periodontal surgery when treating Cyclosporin-induced gingival hyperplasia. Sahika lgaku（J Osaka Odontol Soc) 1996 Sept; 59(3): 252− 258.

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