The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–1,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes.

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A methanol extract of the flowers of Mammea siamensis (Calophyllaceae) was found to inhibit enzymatic activity against aromatase (IC₅₀=16. µg/mL). From the extract, two new geranylated coumarins, mammeasins (1) and D (2), were isolated together with seven coumarins: 8-hydroxy--methyl--(3,-dimethyl-octa-2,6-dienyl)--(2-methyl-1-oxobutyl)-,-dihydropyrano[,3,2-de]chromen-2-one (), 8-hydroxy--methyl--(3,-dimethyl-octa-2,6-dienyl)--(3-methyl-1-oxobutyl)-,-dihydropyrano[,3,2-de]chromen-2-one (10), mammeas A/ (14), A/AB (15), A/ cyclo D (18), / (23), and /BC cyclo D (25). The structures of 1 and 2 were elucidated on the basis of spectroscopic evidence. Among the isolates including 17 previously reported coumarins, 1 (IC₅₀=2. µM), 2 (3.6 µM), and mammea /AB cyclo D (21, 3.1 µM) showed relatively strong inhibitory activities comparable to the activity of the synthetic nonsteroidal aromatase inhibitor aminoglutethimide (2. µM).

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The gamma-ray spectrum and the conversion-electron spectrum are measured in the decay of the ^116mIn activity. Directional correlations are also measured for 818–1293 and 1097–1293 keV cascades. The M1-2 mixing ratio δ, and the -2 mixing ratio μ_k are obtained for 818. keV +→2₁⁺ transition to be δ=1.52−

0.22

+

0.26

, and μ_k≤6.1×10−

4

respectively. The

E

2 branching ratio

B

(

E

2;

2₂

+→

0_g

+):

B

(

E

2;

2₂

+→2₁⁺):

B

(

E

2;

2₂

+→0₁⁺) is determined to be

0

.0158:1.

0

:

5

.86.

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Circuit techniques to enhance the linearity of input-voltage-to-current (V/I) conversion and to increase the output impedance of a current source by compensating for the low intrinsic gain of a transistor were introduced to realize a high-frequency operational transconductance amplifier (OTA) for a low supply voltage using sub-100-nm CMOS processes. Applying these techniques, a MOS th-order Gm- linear-phase low-pass filter (LPF) was realized using a 65nm CMOS process. A simplified biquad LPF that can serve as a component of a th-order LPF was newly developed by replacing OTAs with resistors. As a result, the -3dB frequency bandwidth, group delay ripple, 3rd-order distortion, and 3rd-order input intercept point (IIP3) were 200MHz, 2.2%, ≤ -55dB with a 100MHz input, and +10.3dBm, respectively, all with a ±.1Vp-p input signal at each input terminal in the pseudodifferential configuration. The LPF including an output buffer dissipated 60mW in the case of a 1.2V supply. Wide spurious-free dynamic range (SFDR) characteristics were confirmed up to high frequencies.

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Various serum lipid levels (hypo-, normo- and hyper-cholesterolemic) are shown in dys-β-lipoproteinemia (-LP). Though type III hyper-LP (hypercholesterolemic dys-β-LP) is characterized by abnormal lipid composition of VLDL and elevaied level of apolipoprotein (apo ), diagnosis of normo- and hypo-cholesterolemic dys-β-LP are offenly difficult. At the present study, we established simple and sensitive methods to diagnose dys-β-LP only by measurement of serum apolipoproteins. In purpose to define new approach for diagnosis of dys-β-LP, serum apo , apo -II, apo -III and apo levels were determined by the method of single radial immunodiffusion. These apolipoproteins were analysed in subjects with dys-β-LP (n=), hyper-LP (n=67) and normo-LP (n=41). A serum apo /apo -III ratio in dys-β-LP was 1.37±.59 (mean±SD). This value was significantly higher than the ratios among normo-LP (.45±.17), type IIa (.51±.11), type IIb (.45±.17), type IV (.45±.13) and type V (.47±.). A serum apo /apo (×100) ratio in dys-β-LP was 25.2±10.2. This value was also significantly higher than the ratios among normo-LP (.±1.), type IIa (.±.), type IIb (.±1.), type IV (.2±3.1) and type V (15.±.). Based on these data, it is proposed that the apo /apo -III ratio of >1. was diagnostic value of type III and the apo /apo (×100) ratio of >10. was also diagnostic value of normo- and hypo-cholesterolemic dys-β-LP. As to determine serum apolipoproteins very simple by single radial immunodiffusion method, the ratios will be useful for diagnosis and detection of dys-β-LP in clinical and epidemiological studies.

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We screened the differentiation-inducing activities of 39 mushroom extracts from Akita prefecture, Japan, on the mouse osteoblastic cell line, MC3T3-1. Sixteen phosphate buffered saline (PBS), 8 boiled PBS, 14 ethanol and 12 methanol extracts induced alkaline phosphatase (ALP) activities, an indicator of MC3T3-1 cell differentiation. The enzyme activities were markedly induced by extracts of Tricholoma auratum, and we isolated the active compound from methanol extracts of this mushroom. Physical data for the isolated active compound were identical to those for (,24R)-ergosta-,-diene-3β,,6β-triol (1). 1 induced ALP activities of MC3T3-1 cells and promoted cell proliferation. To investigate the relationships between the chemical structure and differentiation-inducing activity of the compound, ALP-inducing activities of MC3T3-1 cells by 1, ergosterol (2), ergocalciferol (3), cholesta-3β,,6β-triol (), -dehydrocholesterol () and cholecalciferol (6) were tested. The enzyme activities of MC3T3-1 cells were increased 3.-fold by 10 μM 1 and 2.-fold by 10 μM . However, 2, 3, and 6 did not induce MC3T3-1 cell ALP activity at .1—10 μM. These results suggested that the OH groups at - and/or -6 of 1 and played an important role in their differentiation-inducing activities on MC3T3-1 cells. Furthermore, 1 suppressed induction of MC3T3-1 cell apoptosis by serum starvation.

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The gamma-ray spectrum and the conversion-electron spectrum are measured in the decay of the Tb¹⁵² activity. The L-subshell electron lines of the 586. keV +→2₁⁺ transition are separated by the ion-free beta-ray spectrometer. The M1-2 mixing ratio, δ², and the dimensionless ratio X of -2 mixing of the 586. keV transition are obtained to be δ²≤.16 and .034≤X≤.038, respectively. The 2 branching ratio (2; +→+): (2; +→2₁⁺): (2; +→0₁⁺) is determined to be .023 : 1. : .6.

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The phase diagrams of the Ni-Mo- and Ni-W- ternary systems in the region of less than 50mol% were constructed by thermodynamic calculation, based on the data obtained by thermodynamic measurement of the related materials. We found three ternary eutectic points and three or two ternary peritecto-eutectic points as follows:
:L (1365K, 71.mol%Ni-6.mol%Mo-.mol%)=(Ni)++
:L (1355K, 62.mol%Ni-2.mol%Mo-30.mol%)=++
:L (1445K, 42.mol%Ni-30.6mol%Mo-10.3mol%)=(Ni)+NiMo+
P₁:L (1812K, 34.mol%Ni-42.3mol%Mo-.8mol%)+MoB=+
P₂:L (1633K, 42.3mol%Ni-40.mol%Mo-17.3mol%)+Mo=+
P₃:L (1812K, 53.mol%Ni-33.mol%Mo-12.8mol%)+Mo=NiMo+
:L (1622K, 51.mol%Ni-31.6mol%W-17.mol%)=(Ni)+W+
:L (1260K, 71.mol%Ni-.mol%W-.mol%)=(Ni)++
:L (1291K, 65.mol%Ni-.8mol%W-29.8mol%)=++
P₁:L (2115K, 23.8mol%Ni-43.1mol%W-33.1mol%)+WB=+
P₂:L (1657K, 48.mol%Ni-33.1mol%W-18.mol%)+=W+
The calculated phase diagrams are expected to be useful for the development of new Ni-based heat-, corrosion- or wear-resistance alloys.

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[1] The purpose of this reserch is to make clear what type of design can give good effects to win the high readership score. At the begining I had to know what factors decide the readership score, and how to change the quality of the desing into numerical value. I have used the method of multi-dimentional analysis with the help of a computer, and got the estimation formula, with kinds of factors, as follows ; A^^〜=++D+K+aE+bF+cG. A^^〜=estimated values of the readership score. 1) , , D, K : these four factors are given as category values (so we call them); each factor is classified into several categories. =week day, classified into categories. =pages of a newspaper, which are characterized by the kinds of articles, news, and the other reading matters, classified into categories. D=kinds of goods and trades of the advertizement, classified into 14 catedories. K=constants, given to each 13 surveys. 2) , F, G : these factors are given as numerical values. G=area of advertizement, measured by the unit of column in the whole page length, being classified into 6 kinds of area (2., 3., , , 10, 15) and 15 column means a whole page, about 2,000cm^2. , F are the marks obtained in the design of advertizements. =sum of the marks obtained in each design element on the advertizement. F=the marks obtained on the whole effect of the design. 3) a, , : coefficients Using the above formula with the category values and the coefficients, we can obtain the naked design effect from the actual value A of readership score: aE+bF=A-(++D+K+cG). In this research, I used 1,829 data of readership score from 1960 to 1968, being obtained by 13 survevs, spring and autumn twice a year. The sample of size each survey was 3,541, 2,251, or 200 in the other 11 surveys. Multiple correlation coefficient between the estimated value A^^〜 and the actual value A is .951. The table 2^* shows the contribution indexes of each factor by 3 kinds of expression-(1) Range : the absolute difference between the maximum and the minimum category values, (2) Standard deviations of the category values, (3) Partial correlation coefficient : the relationship between the actual values A and each factors. *see the table 2 in the thesis in Japanese. [2] The important point was in the determination of the values of , F. is the sum of the marks obtained in each design element, classified into four kinds : =_1+_2+_3+_. design elements [table] _1, _2, _3, _, these values have the grades, as , 1, 2, 3, and is given to the design that has no attractive effect or no applicable element. And obtain the marks of to grades as the sum of them. The values of F have also grades, to . The principles to determine the values of , F are as follows : a) The marks obtained of , F must be the relative values among the each survey, and at the same time, they must have constancy within the same survey-the same elements of design must win the same marks obtained. ) They must be determined as to win the highest multiple correlation coefficient, when they are put into the estimation formula. ) They must be reasonable. In order to justify them, we must carry many researches on the actual condition. The frequency of accurence of each grade of , F values, as the table -, and - in the thesis in Japanese.

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Nine isoquinoline alkaloids belonging to the protoberberine, proaporphine and bisbenzyl-isoquinoline series were isolated from Caryomene olivascens (Menispermaceae). Four compounds are new : (-)-2-norlimacine (), (-)-caryolivine (6), (+)-1, 2-dehydro-2-norlimacusine () and N-formylstepharine (3). The last one is the first N-formyl derivative to be reported in the proaporphine group. Its biogenetic origin is discussed. Structures were established on the basis of 360 MHz (FT) nuclear magnetic resonance measurements and also by chemical correlation in the case of 3.

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