Isolation of Pure Individual Fatty Acids from Chicken Skin Using Supercritical CO₂ Extractor or Cooling Centrifuge

抄録

Chicken skin -a poultry meat industries waste- has been used in this work as a source for the production of pure free fatty acids. Chicken skin fat was extracted using dry rendering method. Physical and chemical parameters of that fat were determined. Also, its fatty acids composition has been identified by GC-MS after its esterification as oleic, palmitic, linoleic, stearic, myristic, lauric, linolenic, behenic, arachidonic, arachidic, palmitoleic, and paullinic acids, and others as traces. The extracted fat was then hydrolyzed into mixture of free fatty acids and glycerol, the free fatty acid mixture was separated, then it was cooled in order to separate saturated and unsaturated fatty acids from each other. Oleic, Palmitic, Linoleic and Stearic Acids were extracted individually in pure form using supercritical CO₂ extractor. Moreover, oleic, linoleic, palmitoleic, linolenic, and paullinic acids were extracted individually in pure form using cooling centrifuge sigma 3-18KS. All of the separated individual fatty acids were confirmed according to their melting point, GC-MS after esterification, elemental analysis and mass spectrometry (ms) of the corresponding methyl ester in order to detect the corresponding molecular ion peak. Therefore, these new two methods could afford the very expensive pure fatty acids with a low cast.