Effect of trans-Octadecenoic Acid Positional Isomers on Tumor Necrosis Factor-α Secretion in RAW264.7 Cells

Aya Yoshinaga-Kiriake; Kazuaki Yoshinaga; Naohiro Gotoh

doi:10.5650/jos.ess20141

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Effect of trans-Octadecenoic Acid Positional Isomers on Tumor Necrosis Factor-α Secretion in RAW264.7 Cells

Aya Yoshinaga-Kiriake, Kazuaki Yoshinaga, Naohiro Gotoh

著者情報

キーワード: trans fatty acid, inflammation, octadecenoic acid isomer, RAW264.7, TNF-α

ジャーナルフリー早期公開

論文ID: ess20141

DOI https://doi.org/10.5650/jos.ess20141

この記事には本公開記事があります。

The final version of this article is now available: Vol. 69 (2020), No. 9 pp. 1139-1143

詳細

抄録

We compared the cytotoxic effects and tumor necrosis factor-α (TNF-α) production induced by 13 trans-octadecenoic acid positional isomers (trans-4-C18:1 to trans-16-C18:1) in RAW264.7 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and enzyme-linked immunosorbent assay, respectively. No significant differences were observed in the cytotoxic effects among the 13 trans-C18:1 positional isomers and control on RAW264.7 cells. TNF-α production significantly decreased by treatment of trans-4-C18:1 as compared to control, but no significant differences in TNF-α production were observed among other trans-C18:1 positional isomers and control. These results suggest that the double bond position in trans-C18:1 may affect TNF-α production in cells.

責任著者(Corresponding author)

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