シリーズ—研究小集会（第25回）卵部会
加熱変性リゾチームによるノロウイルスの不活化作用について
2016 年 63 巻 8 号 p. 358-362
詳細
2016 年 63 巻 8 号 p. 358-362
Human norovirus (HuNV) infects humans through the consumption of contaminated food, contact with the excrement or vomit of an infected person, and via airborne droplets. Being highly infectious and highly viable in the environment, inactivation of norovirus requires an extremely effective inactivating agent. In this study, we characterized the norovirus-inactivating effects of thermally denatured lysozyme. We observed that lysozymes heat-treated for 40 min at 100°C caused a 4.5 log reduction in the infectivity of norovirus. Transmission electron microscope analysis showed that viral particles exposed to heat-denatured lysozymes were expanded compared to those before exposure. Moreover, we confirmed breaking of the capsid proteins of murine norovirus and HuNV by real-time PCR combined with propidium monoazide.
