Purpose: The purpose of this study was to assess the bactericidal effects of a new silver-based antimicrobial glass DL-7900 on the genus Candida, to clarify the bactericidal mechanism, and to investigate its application to denture cleansers.
Methods: 1) Disk susceptibility tests of DL-7900 for Candida species and the microbroth dilution method for determining Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of DL-7900 for Candida albicans (C. albicans) were performed. 2) The time-dependent bactericidal effects of DL-7900 on C. albicans and the limitations on its number of times of use were investigated. 3) The bactericidal mechanism of DL-7900 on C. albicans was verified by PCR.
Results: 1) All Candida strains used in this study were sensitive to DL-7900, and MIC and MBC of DL-7900 for C. albicans were 3.9 mg/ml and 31.25 mg/ml, respectively. 2) C. albicans treated by DL-7900 for 60 minutes died out completely. The bactericidal effect of DL-7900 on biofilm-forming C. albicans was higher than that of commercial denture cleanser. Moreover, even if DL-7900 was used consecutively, the bactericidal effect did not decrease. 3) It was found that the bactericidal mechanism of DL-7900 on C. albicans was bacteriolysis.
Conclusions: Because DL-7900 showed high and long-term bactericidal activity, this material might be superior as a denture cleanser.
View full abstract