1. The purpose of the present investigation is to secure the basis for rational treatment in manuring and harvesting tobacco plants.
2. The material for this investigation is a negative sort of cultivated tobacco, called “Hatanoshu”. The experiments were conducted through five growth periods with fourteen kinds of sampling, thus covering the whole developmental stages of the plant concerned, viz.
A. Seeds.
B. Seedlings (17 days after sowing and 10 days after germination.)
C. Young plant, suitable for transplanting (56 days after germination.)
D. Plant in the stage of topping (77 days after transplanting.)
a. Leaves. b. Stem. c. Root. d. Flower stalk.
E. Mature plant, suitable for harvesting (103 days after transplanting.)
a. Lower leaves (so-called “Doha” or “Lugs”.)
b. Sub-principal leaves (so-called “Chiiha” or “Cutter”.)
c. Principal leaves (so-called “Hompa” or “Leaves”.)
d. Upper leaves (so-called “Tempa” or “Lips”.)
e. Stem.
f. Root.
g. Flower stalk and axillary buds.
3. Analytical determinations of the organic constituents were carried out after the methods of Kissling and also of Schlösing. The results were given for the following items: the weights of fresh and dry matter; the amounts of total-N, ammonia-N, nitric acid-N, amido-N, protein-N, nicotine-N, malic acid, citric acid, oxalic acid, acetic acid, tannin, pectinic matter, starch, glucose, succrose, dextrin, crude fibre and crude fat, each in percentages of fresh and dry weights of above mentioned samples. Calculations were also made for the content of these constituents in each one plant.
4. The most interesting result was obtained concerning the variation of nitrogenous compounds. Some peculiar features were also observed in the variations of such non-nitrogenous constituents as pectinic matter, organic acids, tannin, sugars, starch, dextrin and fibre.
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