Biological and Pharmaceutical Bulletin

おすすめ記事

42 巻 (2019) 2 号 p. 273-279

Hepatic and Intrahepatic Targeting of Hydrogen Sulfide Prodrug by Bioconjugation

Kosuke Sakai, Hidemasa Katsumi, Kentaro Kamano, Kiyo Yamauchi, Ayuko Hajima, Masaki Morishita, Toshiyasu Sakane, Akira Yamamoto

Hydrogen sulfide (H₂S) is an endogenous gaseous transmitter known to play an important role in biological functions. For the hepatic and intrahepatic targeting of H₂S prodrug at the cellular level, we developed two types of sulfo-albumins, in which five sulfide groups (source of H₂S) were covalently bound to succinylated (Suc) or galactosylated (Gal) bovine serum albumin (BSA). Sulfo-BSA-Suc and polyethylene glycol (PEG)-Sulfo-BSA-Gal, both released H₂S in the 5 mM glutathione solution, but not in the plasma. Sulfo-BSA-Suc and PEG-Sulfo-BSA-Gal were taken up by RAW264.7 cells (mouse macrophage-like cells) and Hep G2 cells (human hepatocellular carcinoma cells), respectively, and H₂S was released. These results indicate that Sulfo-BSA-Suc and PEG -Sulfo-BSA-Gal selectively released H₂S intracellularly. In a biodistribution study, up to 80% of ¹¹¹In-labeled Sulfo-BSA-Suc and PEG-Sulfo-BSA-Gal rapidly accumulated in the liver, 30 min after intravenous injection in mice. Furthermore, ¹¹¹In-labeled Sulfo-BSA-Suc and PEG-Sulfo-BSA-Gal predominantly accumulated in liver nonparenchymal (endothelial cells and Kupffer cells) and parenchymal cells (hepatocytes), respectively. These findings suggest that targeted delivery of H₂S prodrug to a specific type of liver cells was successfully achieved by bioconjugation.

編集者のコメント

The delivery of hydrogen sulfide (H2S) to liver is expected for the treatment of hepatic diseases. K. Sakai et al. developed two types of sulfo-albumins, macromolecular H2S prodrugs, for hepatic and intrahepatic targeting of H2S. Sulfide groups (source of H2S) were covalently bound to succinylated (Suc) and galactosylated (Gal) bovine serum albumin (BSA) for targeted delivery of H2S to hepatic nonparenchymal cells and parenchymal cells, respectively. Their results demonstrated targeted delivery of H2S prodrug to a specific type of liver cells using the chemical modification of targeting ligands.

過去のおすすめ記事を見る

2019 年 42 巻 2 号

Zhongfenggao Protects Brain Microvascular Endothelial Cells from Oxygen–Glucose Deprivation/Reoxygenation-Induced Injury by Angiogenesis Shenghui Huang, Ting Gong, Tengfei Zhang, Xinfeng Wang, Qianqian Cheng, Yanyi Li
Preventive Effect of Citrus aurantium Peel Extract on High-Fat Diet-Induced Non-alcoholic Fatty Liver in Mice Hyoung-Yun Han, Sung-Kwon Lee, Bong-Keun Choi, Dong-Ryung Lee, Hae Jin Lee, Tae-Won Kim
Optimization of Individual Pharmacotherapy Based on Multiple Evaluations of Patient Data Takafumi Naito
Resistance of Fetal Guinea Pig Ventricular Myocardium to Hypoxia: Maintained Intracellular ATP Prevents the Opening of ATP-Sensitive Potassium Channels Naoki Agata, Yoshimitsu Kato, Shogo Hamaguchi, Iyuki Namekata, Hikaru Tanaka

最新号のすべての記事を見る

月間アクセス数ランキング 2019年01月

1

Total Purine and Purine Base Content of Common Foodstuffs for Facilitating Nutritional Therapy for Gout and Hyperuricemia

公開日: 2014/05/01 | 37 巻 5 号 p. 709-721

Kiyoko Kaneko, Yasuo Aoyagi, Tomoko Fukuuchi, Katsunori Inazawa, Noriko Yamaoka

Views: 3,056
2

Possibility of Exosome-Based Therapeutics and Challenges in Production of Exosomes Eligible for Therapeutic Application

公開日: 2018/06/01 | 41 巻 6 号 p. 835-842

Takuma Yamashita, Yuki Takahashi, Yoshinobu Takakura

Views: 594
3

Selective Androgen Receptor Modulator, YK11, Up-Regulates Osteoblastic Proliferation and Differentiation in MC3T3-E1 Cells

公開日: 2018/03/01 | 41 巻 3 号 p. 394-398

Tomofumi Yatsu, Taichi Kusakabe, Keisuke Kato, Yoshio Inouye, Kiyomitsu Nemoto, Yuichiro Kanno

Views: 292
4

Liposomal Delivery Systems: Design Optimization and Current Applications

公開日: 2017/01/01 | 40 巻 1 号 p. 1-10

Amr Selim Abu Lila, Tatsuhiro Ishida

Views: 251
5

A Novel Strategy to Increase the Yield of Exosomes (Extracellular Vesicles) for an Expansion of Basic Research

公開日: 2018/05/01 | 41 巻 5 号 p. 733-742

Sherif E. Emam, Hidenori Ando, Amr Selim Abu Lila, Taro Shimizu, Masami Ukawa, Keiichiro Okuhira, Yu Ishima, Mahmoud A. Mahdy, Fakhr-eldin S. Ghazy, Tatsuhiro Ishida

Views: 221

お気に入り & アラート

ジャーナルのニュースとお知らせ

Biol. Pharm. Bull. Vol. 41 No. 10
Current Topics: Transporter Research Yields New Discoveries in Life Sciences

前身誌

公衆衛生年報

衛生化学

Journal of Health Science

Journal of Pharmacobio-Dynamics

J-STAGEへの登録はこちら（無料）