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Volume 17 (1992)
- Issue 6 Pages 335-
- Issue 5 Pages 257-
- Issue 4 Pages 209-
- Issue 3 Pages 151-
- Issue 2 Pages 87-
- Issue 1 Pages 1-
Volume 17, Issue 3
Displaying 1-9 of 9 articles from this issue
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Shin-ichi Tominaga1992 Volume 17 Issue 3 Pages 151-155
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSTreatment with 1000 units/ml of marine beta-interferon enhanced an adenylate kinaselike activ-ity and markedly increased the level of L-alpha-phosphatidyl inositol 4-monophosphate in quiescent BALB/c-3T3 cells. The addition of platelet-derived growth factor (22 units/ml) or poly(I).poly(C) (0.3-1 microgram/ml) to the phosphorylation reaction mixture did not alter this interferon action.View full abstractDownload PDF (441K) -
Rika Satake-Ishikawa, Masaharu Ishikawa, Yuji Okada, Makoto Kakitani, ...1992 Volume 17 Issue 3 Pages 157-160
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSRecombinant human granulocyte colony-stimulating factor (rHuG-CSF) produced in Escherichia coil was chemically modified by polyethylene glycol (PEG) of molecular weights 4, 500 or 10, 000. The neutrophils observed at 32 hours after intravenous injection of the rHuG-CSF modified with PEG (4, 500) or PEG (10, 000) to mice were, respectively, 2.5 times and 5 times more than that observed after the injection of the un-modified rHuG-CSF. These results show that the covalent attachment of PEG to rHuG-CSF enhanced its pharmacological activity in vivo and that the modification with the larger PEG molecule is more effective to enhance the in vivo activity of rHuG-CSF.View full abstractDownload PDF (327K) -
Kazunori Ohki, Ariaki Nagayama, Osamu Kohashi1992 Volume 17 Issue 3 Pages 161-167
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSWe examined the responsiveness of two factor-dependent macrophage cell lines, BDM-1 and its subclone, BDM-1W3, to bacterial lipopolysaccharide (LPS), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) for their growth. LPS inhibited the M-CSF-dependent proliferation of BDM-1 cells but it had no effect on the proliferation of BDM-1W3 cells. LPS promoted DNA synthesis and supported the cell viability in the absence of CSFs in BDM-1 and BDM-1W3 cells, suggesting that the intracellular signals are transduced from the interaction of LPS with LPS-binding sites in BDM-1W3 cells as well as in BDM-1 cells. IFN-γ inhibited the proliferation of BDM-1 and BDM-1W3 cells. However, BDM-1 cells were more susceptible to the inhibitory effect of IFN-γ than BDM-1W3 cells. In contrast to LPS and IFN-γ, TNF-α did not inhibit the proliferation of BDM-1 and BDM-1W3 cells. These cell lines should be useful for studying the regulatory mechanisms in CSF-dependent macrophage proliferation.View full abstractDownload PDF (625K) -
Satoru Ozono, Minoru Onozuka, Kazuyoshi Sato, Yumi Ito1992 Volume 17 Issue 3 Pages 169-175
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSImmunohistochemical analyses of estradiol, progesterone and progesterone receptor were carried out in human salivary gland and salivary adenoid cystic carcinoma. Immunoreactivity to estradiol and progesterone was found in cytoplasm of the cells of the excretory duct system within normal salivary glands, whereas the progesterone receptor was restricted to nuclei of the cells where both sex steroids were positive. In addition, we demonstrated the presence of both sex steroids and the receptor for progesterone in salivary adenoid cystic carcinomas. These data indicate that the human salivary gland is one of the target tissues of estrogen. This also suggests the good possibility that tumors which express progesterone receptors will respond to endocrine therapy.View full abstractDownload PDF (1330K) -
Akihiro Umezawa, Jun-ichi Hata1992 Volume 17 Issue 3 Pages 177-184
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSBone marrow stromal cells are requisite for the proliferation of hematopoietic cells and communicate with each other via gap junctions. Marrow stromal cells expressed connexin 43, but not connexin 32. H-1/A, a murine marrow stromal cell line, underwent adipocyte differentiation at confluence, and expressed the 3.0 kilobase mRNA species of connexin 43 before differentiation. H-1/A cells studied with the dye-transfer method showed gap-junctional communication with adjacent cells but lost this communication during differentiation. The connexin 43 transcripts in H-1/A cells were down-regulated before the expression of glycero-monophosphate dehydrogenase was induced. Loss of gap-junctional communication was regulated at the mRNA level of connexin 43. Connexin 43 expression was down-regulated at the transcriptional level.View full abstractDownload PDF (1416K) -
Sayumi Shibamoto, Makio Hayakawa, Takamitsu Hori, Naoto Oku, Keiji Miy ...1992 Volume 17 Issue 3 Pages 185-190
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSHepatocyte growth factor (HGF) induced scattering and cell migration of human gastric adenocarcinoma MKN-74.HGF also significantly promoted the growth of MKN-74 cells in a dose-dependent manner, although HGF is reported to be antiproliferative for the growth of tumor cell lines. This result indicates that HGF stimulates cell proliferation of not only normal epithelial cells but also certain carcinoma cells. Furthermore, transforming growth factor-β(TGF-β), which is recognized to inhibit the growth of most epithelial cells, additively enhanced both the cell proliferation and migration induced by HGF. These additive effects of HGF and TGF-β may be responsible for the tumor invasiveness and uncontrolled growth of certain types of carcinoma.View full abstractDownload PDF (833K) -
Chieko Mineo, Toku Kanaseki, Tsuneo Imanaka, Tatsuya Takano1992 Volume 17 Issue 3 Pages 191-196
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSThe mechanisms of hydrolysis and accumulation of cholesteryl oleate-lipid droplets prepared in vitro were studied in acid cholesteryl-esterase-defident fibroblasts (GM00863, GM03111).
Acid cholesteryl esterase activity was reduced in both GM00863 and GM03111 (S.9% and 17.4% of the normal level, respectively), while neutral cholesteryl esterase activity was highly stimulated in GM03111. The hydrolysis of [14C]-cholesteryl oleate-lipid droplets in GM00863 was almost as efficient as in normal cells, while that in GM03111 was highly stimulated. Whenviewed by polarized microscopy the lipid droplets which had accumulated in the mutant cells showed anisotropic liquid crystalline structures. As in normal cells, some of these lipid droplets were observed by transmission electron microscopy as membrane-free lipid inclusion bodies in the cytoplasm. These results suggest that lipid droplets internalized into phagolysosomes of these mutant cells transferred to the cytoplasm, and were hydrolyzed there probably by neutral cholesteryl esterase.View full abstractDownload PDF (1242K) -
Kazuhiro Ishii, Hirokazu Inoue, Akira Hakura, Wataru Araki, Tohru Masu ...1992 Volume 17 Issue 3 Pages 197-202
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSFetal mouse brain glial cells in primary cultures were immortalized by recombinant retroviruses containing human papillomavirus type 16 E7 genes, and named VR-2g cells. The presence and expression of E7 genes in VR-2g cells were demonstrated by the Southern and the Northern blot analyses. VR-2g cells did not form colonies in soft agar culture, indicating that VR-2g cells have no transforming phenotypes. By the karyotype analysis, VR-2g cells consisted of two cell populations, the pseudo-diploid and the pseudo-tetraploid. VR- 2g cells were positive in immunostaining with mono- and polyclonal antibodies against glial fibrillary acidic proteins (glial-specific intermediate filaments). In addition, VR-2g cells secreted neurotrophic factors as assayed with primary cultures of fetal rat striatum neurons, although molecular characterization of the factor(s) was not yet determined. These results indicate that the present method for cell immortalization will be useful for establishing untransformed cell lines from primary cultures of fetal brain cells.View full abstractDownload PDF (1495K) -
Naomi Kamasawa, Nobuko Naito, Tatsuo Kurihara, Yasushi Kamada, Mitsuyo ...1992 Volume 17 Issue 3 Pages 203-207
Published: 1992
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSThe location of acetoacetyl-CoA thiolase (T-I) and 3-ketoacyl-CoA thiolase (T-III), enzymes of the fatty acid β-oxidation system, was studied in n-alkane-grown Candida tropicalis cells by immunoelectron microscopy using a post-embedding method with colloidal gold conjugated IgG. The deposition of gold particles for T-I was detected in the microbodies and cytoplasm and that of gold particles for T-III specifically in the microbodies. The double labeling technique confirmed that T-I and T-III occurred concurrently in a microbody and T-I also in cytoplasm. These results were consistent with the biochemical data based on subcellular fractionation and indicated that the yeast β-oxidation system operates efficiently only in the microbodies.View full abstractDownload PDF (2000K)
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