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Volume 15 (1990)
- Issue 6 Pages 323-
- Issue 5 Pages 237-
- Issue 4 Pages 175-
- Issue 3 Pages 127-
- Issue 2 Pages 73-
- Issue 1 Pages 1-
Volume 15, Issue 3
Displaying 1-7 of 7 articles from this issue
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Keiji Tanaka, Akira Ichihara1990 Volume 15 Issue 3 Pages 127-132
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSDownload PDF (1135K) -
Susumu Hirose, Tsutomu Ohta1990 Volume 15 Issue 3 Pages 133-135
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSDownload PDF (198K) -
Yasuo Tanno, Yuriko Shindoh, Tamotsu Takishima1990 Volume 15 Issue 3 Pages 137-142
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSThe effects of T cell factors, including interleukin (IL)-3 and IL-4, and fibroblasts on the growth and differentiation of human lung mast cells (MCs) obtained by bronchoalveolar lavage (BAL) were examined. The numberof MCsidentified by alcian blue-safranin staining was twice that of the control culture without conditioned medium (CM) when BALcells were cultured for 2 weeks in RPMI 1640 containing 10% fetal calf serum and partially purified CMderived from PHA-stimulated lymphocytes.
In the presence of both recombinant (r) IL-3 and rIL-4, the number of MCswas twice as high as the control without increase in the per-cell histamine content after 2 weeks' culture. In umbilical cord blood cultures, IL-3 plus IL-4 augmented basophilic cells about 20-fold more than the control when cultured for 2 weeks. In some cases, the percentage of safranin-positive MCswas about 2-5 fold greater, with 2-7 fold higher histamine content, when cultured for 10 days with CMand fibroblasts derived from human embryonic lung. However, in all BALexperiments, there was no increase in the total numberof MCsafter culture compared with the initial number of MCs, unlike the umbilical cord blood cultures. These results suggest that T cell factors, including IL-3 and IL-4, and fibroblasts may influence the phenotype and the survival of lung mast cells in BAL, whereas there was no evidence for the presence of MCprecursors in BALfluids.View full abstractDownload PDF (485K) -
Kazuto Ohkura, Takeru Fujii, Ryoji Konishi, Hiroshi Terada1990 Volume 15 Issue 3 Pages 143-150
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSIn culture epidermal cells from the skin of newborn rats became attached to Millipore filters coated with type IV collagen much better than to filters coated with type I collagen. Ascorbic acid markedly increased the attachment and viability of epidermal cells seeded on type I collagen, but had no significant effect on cells seeded on type IV collagen. It was also found to enhance the synthesis of type IV collagen by the cells, which, we concluded, enabled the cells to become well attached to type I collagen.
This conclusion was supported by studies on the penetration of trypan blue through the cell layers. There was a lag in penetration through cell layers cultured both with and without ascorbic acid on Millipore filters coated with either type I or IV collagen, indicating that the cells were con fluent over the whole surface of the filters. The lag was muchlonger in the cultures with ascorbic acid, indicating greater confluence and tighter attachment of cells due to production of type IV collagen. The penetration was found to be due to destruction of the con fluent cell layers by its cytotoxic effect. The time lag before penetration of trypan blue is a good index of the confluence and attachment of cultured cells to collagen layers.View full abstractDownload PDF (1629K) -
Martin Poot, Katrin Kausch, Julia Köhler, Thomas Haaf, Holger Hoe ...1990 Volume 15 Issue 3 Pages 151-157
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSDistamycin A, netropsin and berenil are knownto cause undercondensation of heterochromatic regions of metaphase chromosomes. These ligands interfere with DNA curvature by binding to the minor groove of the DNA. Whereas the effects of these ligands upon chromatin structure are well established, little is known about their possible interference with cell cycle progression. We show that the presence of these DNA ligands causes protracted cell growth consisting of a prolongation of the Gl phase of the cell cycle along with arrest in the G2 compartment. Concomitant with these cell kinetic disturbances the DNA ligands cause increased polyploidisation. These observations suggest that the DNA-minorgroove may play an important role in progression through the G2phase and proper mitotic transit.View full abstractDownload PDF (578K) -
Yukihisa Hamaguchi, Miyako S. Hamaguchi1990 Volume 15 Issue 3 Pages 159-162
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSAn increase in intracellular Ca2+ concentration ([Ca2+]) and morphological events were simultaneously observed by epifluorescence and differential interference contrast (DIC) microscopy during fertilization of the sand dollar, Clypeaster japonicus. [Ca2+], which was detected by a Ca2+ indicator, Fluo-3, initially increased just beneath the sperm-attached site on the egg surface 8.6 sec after attachment. The increase spread into the egg as a concentric sphere to the egg center and, thereafter, propagated in the egg cytoplasm as a planar wave rather than a spherical wave. It reached the site opposite the initiation site across the egg 24.2 sec after initiation. The fertilization envelope (FE) began to elevate 10.3 sec after the initiation of the increase in [Ca2+l and 21.2 sec after sperm attachment.View full abstractDownload PDF (1310K) -
Hiroko Otsu, Akitsugu Yamamoto, Nobuaki Maeda, Katsuhiko Mikoshiba, Yu ...1990 Volume 15 Issue 3 Pages 163-173
Published: 1990
Released on J-STAGE: March 27, 2006
JOURNAL FREE ACCESSUltrastructural localization of I11SP3 receptor in mouse cerebellar Purkinje cells was investigated by immunogold technique using three monoclonal antibodies (mab 10A6, 4C11 and 18A10). The epitopes of the three antibodies were numerously detected on the smooth endoplasmic reticulum (ER) (especially, on the stacks of flattened smooth ER, subsurface cisterns and spine apparatus), scantily on the rough ER and on the outer nuclear membrane, but were not detectable on either the plasmalemma, synaptic densities, mitochondria or Golgi apparatus. Not only mab 4C11 and 10A6 which bind to the N-terminal region of the receptor but also 18A10 which binds to the C-terminal region were localized on the cytoplasmic surface of the ER membranes. This indicates that the C terminus of InsP3 receptor is localized on the cytoplasmic surface of the ER. Wenoticed that gold particles are usually localized on the fuzzy structure of the cytoplasmic surface of smooth ER, which is suggested to correspond to the feet structure of the ryanodine receptor. In the Nissl body, gold particles were found not only on the ERmembranes but also in the cytoplasmic matrix between the rough ER cisterns. Wesuggest that the peculiar structure of Nissl body, which is composed of parallel cisterns of rough ER, sandwiching a number of free polyribosomes between the cisternal elements, is due to the fact that the major proteins like
I11SP3 receptor are synthesized mostly on the free polyribosomes and become membrane bound only at the later stage of the biosynthesis.View full abstractDownload PDF (4989K)
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