All issues
Volume 3 (1978)
- Issue 4 Pages 279-
- Issue 3 Pages 145-
- Issue 2 Pages 71-
- Issue 1 Pages 1-
Volume 3, Issue 4
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
Masana Ogata, Junko Mizugaki1978 Volume 3 Issue 4 Pages 279-292
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSAcatalasemia and hypocatalasemia are genetic diseases due to defective or incomplete catalase activity in blood cells and, in some cases, in tissue cells, but acatalasemic cells have some residual catalase. Half of the patients may suffer from severe oral gangrene but rest have no pathologic symptoms. Cells from the patients have glutathione peroxidase and may havenearly normal levels of other hydrogen peroxide decomposing enzymes. These enzymes together with residual catalase may protect the cells from damage from excess hydrogen peroxide, though this has not been verified. The biochemical characteristics of the residual catalases differ among patients from different areas and from those of animals, e.g. the catalase from the Japanese type acatalasemia is similar to that of a normal person, whereas the residual catalases in the Swiss type acatalasemia and the radiation induced mouse acatalasemia differ from those of normal individuals.View full abstractDownload PDF (1324K) -
Masaru Yamaizumi, Mitsuru Furusawa, Tsuyoshi Uchida, Toshikazu Nishimu ...1978 Volume 3 Issue 4 Pages 293-304
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSWhen erythrocytes were fused to cultured cells with HVJ, hemoglobin in erythrocytes was transferred to the cells. The hemoglobin became uniformly distributed in cells within 5 min, but components of the erythrocyte membranes did not become completely incorporated into recipient-cell membranes for about 2 h. Division of recipient cells was normal but did not start until one day after fusion. Human and guinea-pig erythrocytes had the highest fusion capacities of various animal erythrocytes tested. When these erythrocytes and iodinated bovine serum albumin (BSA) were dialyzed against hypotonic solution, BSA was trapped in erythrocyte ghosts within 30 min, at about 50 % of initial concentration in the dialysis bag. Of BSA trapped in ghosts, 80 % was found in the cytoplasmic fraction and 20 % associated with the membrane fraction. BSA was quite stable in these ghosts. Other proteins with various isoelectric points were also introduced into ghosts, and these proteins were transferred to recipient cells by virus-induced cell fusion of these ghosts. About 80 % of recipient cells in either monolayers or suspensions fused with the ghosts. Use of iodinated BSA showed that 0.25 % of the initial amount of BSA in the dialysis bag was introduced into recipient cells.View full abstractDownload PDF (1370K) -
Miyama Ken, Inoue Isao, Yonosuke Kobatake1978 Volume 3 Issue 4 Pages 305-311
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSSingle heart-muscle cells were isolated from chick embryos. The zeta potential of these cells was determined from measurements of the cellelectrophoretic mobility in accordance with the Helmholtz-Smolukowskiequation in a variety of environmental salt compositions. Ca2+, Mg2+ and K+ shifted the zeta potential to a positive direction.View full abstractDownload PDF (664K) -
Hiroshi Miyamoto, Tetsuhiro Sakai, Toshitaka Ikehara, Kenichi Kaniike1978 Volume 3 Issue 4 Pages 313-324
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSThe usefulness of Rb+ was investigated as an analog of K+ in membrane transport and in supporting the growth of HeLa cells. On replacement of extracellular K+ by Rb+, intracellular Rb+ content increased linearly at 15 min. K+ content declined in contrast with Rb+, but Na+ content was unaffected. In Rb+-preloaded cells, Rb+ and K+ were exchanged after replacement of extracellular Rb+ with K. A kinetic analysis of intracellular Rb+ content showed that most K+ was exchangeable with Rb+, but a small part of K+ was not. Both Rb+ and K+ influxes were equally inhibited by ouabain or furosemide. Rb+ efflux was repressed by furosemide, whereas K+ efflux wasnot influenced. When 90 % of K+ was replaced by Rb+ in serum-free chemically defined medium, cell growth and protein synthesis were inhibited. After total replacement of extracellular K+ by Rb+, cell growth ceased and cellular protein content decreased, while a small part of intracellular K+ remained unexchanged. HeLa cells seemed to accumulate Rb+ in preference to K+.View full abstractDownload PDF (1258K) -
Masana Ogata, Tohoru Hasegawa1978 Volume 3 Issue 4 Pages 325-330
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSThe effect of paraquat (a kind of herbicides) on the mitochondrial energy transfer reaction was investigated. Paraquat stimulated state 4 respiration, and it decreased the respiratory control index, by 30 percent at a dose of about 100μM. Paraquat did not release K+ from mitochondria and also did not affect latent mitochondrial ATPase activity, indicating that it has little injurious effect on mitochondrial membrane.View full abstractDownload PDF (478K) -
Shigehito Ishiguro, Hisao Yamaguchi, Yoshikane Oka, Hiroshi Miyamoto1978 Volume 3 Issue 4 Pages 331-340
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSSynchronous L cells obtained by a selection method were used to study changes in oxygen uptake, lactate production, and ATP turnover time during the cell cycle. The oxygen uptake per cell (Q02) fluctuated periodically with the maximum at S phase and the minimum at G1 phase. The rate of lactate production per cell was kept constant during the initial 14 hours (G1 and S phases) and gradually decreased to one-half at 24 hours after reinoculation. The calculated ATP production per unit cell volume (ium3) indicated a value of 10-15 moles per hour during the cell cycle. The ATP turnover time oscillatedevery 15 to 19 seconds during the cell cycle, being shorter in early G1 and S phases. These data suggest that ATP utilization increases in G1 and S phases.View full abstractDownload PDF (884K) -
Akira Sono, Kengo Sakaguchi1978 Volume 3 Issue 4 Pages 341-347
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSVarious antitumor agents were used to investigate the induction of sister chromatid exchanges (SCEs), one of the most sensitive tests for detecting the effects of mutagenic carcinogens. Antimetabolites in nucleic acid synthesis and inhibitors of protein synthesis were tested on Chinese hamster cells in vitro. These agents increased the frequency of SCEs even after a one hour exposure, and during the limited stage of the last cell division cycle beforefixation. In chase experiments, the response curves of SCEs and chromosomeaberrations were somewhat similar, but seemed to fluctuate independent of each other. Chromosome breaks and gaps induced by the agents were not necessarily associated with SCEs at the breakpoints. Possible relationships between SCE formation and chromosome aberrations are discussed.View full abstractDownload PDF (2287K) -
Nori Kurata, Michio Ito1978 Volume 3 Issue 4 Pages 349-356
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSThe site of chromosomal DNA synthesis during zygotene stage of meiosis was explored by electron microscope autoradiography. Zygonema of Lilium longiflorum cultured in the presence of 3H-thymidine for 24 h frequently showed label distribution over the axial cores prior to formation of synaptinemal complexes. When the culture was continued in isotope-free medium, the zygotene label appeared over synaptinemal complexes. Light microscope autoradiographs of cells showed the zygotene label over both paired and unpaired regions of chromosomes at pachytene. The relevance of these observations to the formation of synaptinemal complex was discussed.View full abstractDownload PDF (1185K) -
Motoe Hirata-Hibi, Nobuo Watanabe, Takehiko Tachibana, Michiko Takeda, ...1978 Volume 3 Issue 4 Pages 357-360
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSBlastoid cells were found after initial Ficoll-Isopaque gradient centrifugation for mononuclear cell separation of normal human peripheral leukocytes. Both T and B cells were found in blastoid forms in rosette separations : T cells by neuraminidase-treated sheep red blood cells and B cells by yeast with human complement. Furthermore, the blastoid cell count increased after the second Ficoll-Isopaque gradient centrifugation. No blastoid forms were found concurrently in heparinized and non-heparinized blood in the same subjects. These observations seem to indicate that Ficoll-Isopaque probably induces blastformations in lymphocytes.View full abstractDownload PDF (380K) -
Kinji Inoue1978 Volume 3 Issue 4 Pages 361-365
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSSera from normal rabbits showed strong cytotoxicity against cells of a mouse pituitary tumor cell line. This cytotoxicity was completely inhibited by heat treatment (56°C for 30 min) of rabbit serum (RS), but it was not restored by addition of only guinea pig serum (GS) into heated rabbit serum (HRS). For restration of HRS cytotoxicity, both GS and newborn calf serum (NCS) were needed. It is possible that the RS cytotoxicity was caused by natural antibody of RS against some antigen of the mouse pituitary tumor cell line.View full abstractDownload PDF (607K) -
Ayako Ueki, Fuminori Hyodoh, Tetsuo Kimoto1978 Volume 3 Issue 4 Pages 367-370
Published: 1978
Released on J-STAGE: April 18, 2008
JOURNAL FREE ACCESSABSTRACT. Human and rabbit fibroblasts showed marked phagocytic activity for latex particles coated with human IgG when they were incubated at 37°C in vitro. After 24 to 48 hours of incubation the intracellular acid phosphatase activity increased and release of the enzyme was stimulated. A strong histochemical reaction of acid phosphatase was observed in the cell cytoplasm under a light microscope. Electron microscopy showed that the reaction was localised in the secondary lysosomes, especially in the dense reaction products around the IgG-coated latex particles. The intensity of the acid phosphatase reaction was higher in latex-phagocytized cells than in the non phagocytic controls. This indicates that the activity of acid phosphatase, a typical lysomal enzymes, is stimulated in fibroblasts by phagocytosis as it is in neutrophils and macrophages.View full abstractDownload PDF (471K)
- |<
- <
- 1
- >
- >|