This paper described that relationship between
Acidithiobacillus feroooxidans T23-3 and fungus TEF1-1 in an enrichment culture. In order to evaluate the relationship, some culture experiments using cell-free extracts from T23-3 and TEF1-1 were carried out. Chemolitotrophic iron-oxidizing bacterium,
A. ferrooxidans T23-3, was able to grow using inorganic compounds in cell-free extract of fungus TEF1-1 or
A. ferrooxidans T23-3. Since the fungus TEF1-1 was able to grow using cell-free extract of the both microorganisms at pH 1.5, the fungus used some organic compounds in the cell-free extract as energy and carbon sources. When the cell-free extract of TEF1-1 used for the cultivation of
A. ferrooxidans T23-3, lag-phase was observed because of lack of magnesium ion. Minimum concentration of magnesium ion for complete growth of
A. ferrooxidans T23-3 was found to be 2.0 x 10
-5 mol/dm
3 from the experiments using synthetic medium. Also the cell-free extract contained enough nutritious components except magnesium ion for the growth of
A. ferrooxidans T23-3. It was suggested that fungi grew using organics and inorganic ions released from the cell of chemolithotrophic iron-oxidizing bacteria by bacteriolysis and/or metabolisum and that the bacteria grew using inorganic ions from the lysis of fungi cells in the enrichment culture of iron-oxidizing bacteria. In addition, chemolithotrophic iron-oxidizing bacterium fixes carbon dioxide to convert some organic compounds in the culture. Therefore, it was evaluated that the relationship between chemolithotrophic iron-oxidizing bacteria and fungi was mutualistic symbiosis in the enrichment culture.
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