The Journal of Poultry Science

おすすめ記事

53 巻 (2016) 2 号 p. 157-164

Expression of Prolactin Receptor on the Surface of Quail Spermatozoa

Gen Hiyama, Shusei Mizushima, Mei Matsuzaki, Yoshinobu Ichikawa, Norio Kansaku, Tomohiro Sasanami

Prolactin receptor (PRLR) is expressed in a wide variety of tissues and mediates diverse biological actions of prolactin (PRL). In mammals, PRL signaling is thought to be involved not only in the process of spermatogenesis and steroidogenesis in the testis, but also in the survival of ejaculated sperm. In avian species, although the expression of PRLR with several variants in the testis was reported, the role of PRL in testicular function is still unclear. The aim of this study was to examine the expression of PRLR in the testis and mature sperm in quail. It is revealed that PRLR was mainly localized in the round- and elongated-spermatid by immunohistochemical analysis on the testis suggesting that PRL signaling may participate in the spermatogenesis. Western blot analysis confirmed the presence of PRLR in the plasma membrane of the ejaculated sperm (SPML), whereas the size of PRLR in the sperm was smaller than that in the hypothalamus. Moreover, PRLR was detected on the surface of the midpiece and flagellum of sperm by immunostaining. To evaluate the functionality of the sperm PRLR, the dot blot assay was performed to test the binding of pituitary PRL to PRLR in the SPML, and resulted in the detection of specific binding of PRL to the component of SPML, most likely to sperm PRLR. Furthermore, when the ejaculates were incubated with pituitary PRL to investigate the role of PRL on the sperm, the occurrence of spontaneous acrosome reaction was significantly decreased. In addition, the expression of PRL on the surface of utero-vaginal junction of oviduct was detected by immunohistochemistry. These results may suggest a novel system that the interaction between oviductal PRL and sperm PRLR is involved in the maintenance of the fertilizability of the spermatozoa through the prevention of the spontaneous acrosome reaction in Japanese quail.

53 巻 (2016) 3 号 p. 181-191

Effects of a Whole-grain Paddy Rice Diet on the pH Distribution in the Gizzard and Retention Time of Digesta in the Crop of Broiler Chicks

Mari Nishii, Masaharu Yasutomi, Yoshiaki Sone

We previously reported that a diet containing 65% paddy rice suppressed the colonization of Campylobacter jejuni in the cecum of broiler chicks, suggesting that this type of diet has positive effects on upper gastrointestinal tract function in broilers. To reveal the possible mechanisms involved in this antibacterial effect of the whole-grain paddy rice diet, we performed experiments comparing the digesta passage rate in the crop and gizzard, the development of gizzard, and the pH distribution in the gizzard between groups of chicks fed two different diets, such as ground corn and whole-grain paddy rice. During these experiments, we made the following observations: the chicks in the group fed the whole-grain paddy rice diet had more developed gizzards and significantly larger crop content than the chicks in the group fed the ground corn diet. The chicks fed the whole-grain paddy rice diet retained the digesta in the crop for much longer and had less variation in the pH values in the gizzard than those fed ground corn. On the basis of these observations, we concluded that the hardness of the rice hull in whole-grain paddy rice leads to a larger amount and longer retention of content in the crop, as well as the uniformity of the internal pH of the gizzard, by promoting gizzard activity. We speculate that the hardness of the rice hulls promoted the grinding activity of the gizzard, resulting in the long retention time of the digesta in the crop and uniformity of the internal pH of the gizzard, which may sterilize or suppress Campylobacter growth in the gastrointestinal tract of broiler chicks.

過去のおすすめ記事を見る

55 巻 (2018) 2 号

Gene Effects on Body Weight, Carcass Yield, and Meat Quality of Thai Indigenous Chicken Amonrat Molee, Petladda Kuadsantia, Porntiwa Kaewnakian
Expression of Prolactin Receptor mRNA in Lactotrophs and Somatotrophs of the Chicken Anterior Pituitary Gland Hiromi Kaneda, Nobuhiro Nakao, Nobumichi Tsushima, Minoru Tanaka
Proteomic Analysis of Thermal Regulation of Small Yellow Follicles in Broiler-Type Taiwan Country Chickens Chuen-Yu Cheng, Wei-Lin Tu, Chao-Jung Chen, Hong-Lin Chan, Chih-Feng Chen, Hsin-Hsin Chen, Pin-Chi Tang, Yen-Pai Lee, Shuen-Ei Chen, San-Yuan Huang
A Study on Growth Performance of Ducks Fed Diets with Different Types of Sipjeondaebo-Tang Byproduct Meal and Red Ginseng Marc with Fermented Red Koji and Ammonia Fluxes in Duck Litter using Alum or Aluminum Chloride Tae-Ho Chung, Chang-Man Kim, In-Hag Choi

最新号のすべての記事を見る

月間アクセス数ランキング 2018年04月

1

A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels

公開日: 2014/07/25 | 51 巻 3 号 p. 307-312

Yutaka Tahara, Katsuya Obara
2

Factors Affecting Egg Internal Quality and Egg Shell Quality in Laying Hens

公開日: 2004/12/03 | 41 巻 3 号 p. 161-177

Juliet R. Roberts
3

Effects of a Protein Kinase Inhibitor on Sperm Motility in the Japanese Quail

公開日: 2017/01/25 | 54 巻 1 号 p. 73-79

Mei Matsuzaki, Shusei Mizushima, Yoshinobu Ichikawa, Kogiku Shiba, Kazuo Inaba, Tomohiro Sasanami
4

Effects of Various LED Light Colors on Growth and Immune Response in Broilers

公開日: 2016/01/25 | 53 巻 1 号 p. 76-81

Hye-Suk Seo, Min Kang, Ran-Hee Yoon, Jae-Hee Roh, Bai Wei, Kyeong Seon Ryu, Se-Yeoun Cha, Hyung-Kwan Jang
5

Effect of Feeding Diets Combining Whole-Grain Paddy Rice and High Levels of Fat on Broiler Chicken Growth

公開日: 2016/01/25 | 53 巻 1 号 p. 34-39

Fumika Nanto, Chiaki Ito, Motoi Kikusato, Masaaki Toyomizu

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