[in Japanese]

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With the purpose of obtaining detailed information about the growth of corpus luteum after the rupture of the follicle (ovulation) in milk cows, 64 cases of ovarian cycle were clinically observed in 9 cows (Holstein) of 2 years and 9 months6 years and 6 months old. In 16 of 64 cases liquid in the antrum of corpus luteum was recognized by rectal palpation. Therefore in this paper we have dealt with the duration of liquid in the antrum after the rupture of follicle, and its influence on the ovarian cycle. The presence of liquid in the antrum was determined by the characteristic form of the corpus luteum and its distortion under the pressure, or by the recognition of the escape of liquid from corpus luteum when pressure was applied. Two of 16 cases were dissected on 7th and 12th day after the rupture of the follicle in order to confirm the results of rectal palpation. Desire, secretion of vaginal mucus and the contraction of the uterus were regarded as the signs of estrus. Results were as follows:
1) In 6 cases (see group I in table 1) the duration of liquid in the antrum of the corpus luteum varied from 7 to 17 days after the rupture of follicle. The presence of liquid was recognized only by the characteristic form of the corpus luteum. In one of 6 cases its ovarian cycle was prolonged for 30 days, but the ovarian cycle of other cases was normal.
2) In 8 cases (see group II in table 1) the duration of liquid in the antrum after the rupture of follicle varied from 2 to 14 days. These variations in the duration of liquid were determined by the recognition of liquid escape from the corpus luteum when pressure was applied. The ovarian cycles of 7 cases were normal and in the other case it was not observed thoroughly owing to the dissection.
3) In 2 cases (see group III in teble 1) the ovarian cycle was not thoroughly observed owing to the dissection.
4) In the formation of corpus luteum which contained liquid in its antrum, two different conditions were distinguishable by rectal palpation. One was derived from the ruptured follicle as usual. The other was derived from the follicle which kept a little liquid in its antrum and maintained the slackness of follicular wall for several days after its rupture, but in this case it is not sure whether the follicle ruptured and the ovum was released or not.
5) The maximum size of corpus luteum was 1625 mm.

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Injections of mammalian gonadotrophin (1100I.U. of PMS or 2.5mg of FSH) into immature pullets caused no appreciable change in the nature of plasma proteins when tested by the NaCl-Ac turbidity test. In all pullets treated with PMS, significant combs growth and marked yellow pigmentation of the leg were observed after 5th injection, while increased oviduct weight was confined to few pullets which exhibited follicular growth in the ovary at the termination of the injection period.
Pullets treated with FSH showed no change either in the plasma proteins or in the organ weight.

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Total plasma protein of hen blood increases with age at a very slow rate until the time of sexual maturity. During this period, nearly the same value of plasma protein can be obtained regardless of the methods of determination. Further, plasma samples taken from immature hens give a negative reaction whenever tested by the NaCl-Ac turbidity test. Approximately 10 days before the day of first oviposition, significant amount of lipophosphoprotein appears in plasma. Its concentration soon reaches to that of laying hen and hens come into lay. After the onset of laying, plasma protein fluctuates in a cyclic fashion without affecting the rhythm of laying.

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This is a result of experiments which confirmed the biological activities of a preparation of sheep ICSH (LH) used in the course of studies on the actions of gonadotrophins.
The effects of relatively large doses of the preparation of ICSH on the reproductive system of hypophysectomized immature female rats were studied.
The significant increase of ovarian weight and the follicular development was not observed in all dosage levels. The interstitial cells were, already, repaired at a dose of 50γ which was the minimal dose adopted in this experiment. No corpus luteum was formed even at a large dose of ICSH (1000 γ). The uterine weight increased, slightly, at doses more than 200 γ.
These results were almost the same as those given by the previous workers who used the pure sheep or porcine ICSH.

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[in Japanese]

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Effects of the combined treatment with the minimal effective dose of purified swine FSH (200γ) and the various levels of purified sheep ICSH (LH) on the ovaries and the uteri of hypophysectomized immature female rats were studied. The experimental conditions were the same as that of the previous report⁵⁾ except that FSH and ICSH were injected separately into the underskin at different sites of the body.
On injection of 200γ of FSH alone, ovaries and uteri were increased in weight. This dose of FSH caused the development of follicles with stimulated internal theca cells and without repair of the deficient interstitial cells. The ovarian and uterine weights were barely increased by the large dose (1 mg) of ICSH. The most conspicuous effect of ICSH was the stimulation of the interstitial tissue. The augmentation of the effect of 200γ of FSH on the increase of ovarian and uterine weights was not conspicuous by combination with ICSH. The histological examination demonstrated that the interstitial tissue was highly stimulated and the follicular growth with the development of internal theca cells was somewhat augmented, but no thecal luteinization nor formation of corpus luteum was detected. The stimulative effect of FSH on estrogen secretion, estimated by the uterine development, was not so increased by the combined treatment of ICSH. The authors (1957) found that FSH is the potent substance to induce ovulation in adult rats⁸⁾.
By these results, it would be said that the main effect of ICSH on the ovary may be the stimulation of interstitial tissue, and FSH might play more important role to cause the formation of corpus luteum.

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