An ischemia-reperfusion injury and the nitric oxide (NO) which thereby occur participate in the outbreak of pressure ulcer. In addition, diabetes participates in the healing delay of the wound, but the influence that a wound in diabetes receives by NO is unknown. In this study, We examined the condition of a patient of the pressure wound in diabetes and the connection with the change of the NO. We repeated liberation with a neodymium magnet three times for pressure, two hours for two hours and manufactured a pressure wound with 12 SD rats 7 weeks of age male. We collected blood at the time of first before pressure and each pressure liberation and measured quantity of nitrogen oxides (NOx). We passed through the pressure wound that manufactured it and observed it for a day and extracted wound after seven days and performed a histological examination. The diabetes group accepted a healing of wound delay in comparison with a control group. In addition, in the diabetes group, quantity of blood NOx significantly showed a low value at all measurement. For a wound healing delay in the diabetes group, the possibility that a bloodstream obstacle to wound region by the relative drop of the NO derived from NOS to act on blood vessel relaxation characteristics was a cause was thought about.