巻号一覧
57 巻 (2009)
- 6 号 p. 371-
- 5 号 p. 331-
- 4 号 p. 241-
- 3 号 p. 117-
- 2 号 p. 45-
- 1 号 p. 1-
57 巻, 4 号
選択された号の論文の11件中1～11を表示しています
- |<
- <
- 1
- >
- >|
一般論文
-
角出 泰造, 豊原 恵, 野町 美弥, 角田 真英, 中田 和彦, 石田 康行2009 年 57 巻 4 号 p. 241-248
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーAs an alternative to commonly used biochemical, morphological, or gene technological analysis, we have evaluated a novel method for rapid classification of microorganism retrieved from used contact lens cases based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Test microorganisms were isolated from contact lens cases. The cases were used daily for lens storage and care for 2 weeks. For this evaluation study, 659 microorganism isolates from the contact lens cases of 16 subjects were analyzed by MALDI-TOF-MS. The mass spectra obtained from the test isolates were compared with authentic spectra of a library database using application software Biotyper 2.0 (Bruker Daltonics). Results show that 644 of 659 (97.7%) isolates were successfully identified and classified into species by the MALDI-based method. Furthermore, these results were interpreted in terms of the risk of infectious disease. Our method is especially useful for simultaneous classification of a large number of test microorganisms because of fewer preparative steps and lower cost.抄録全体を表示PDF形式でダウンロード (1097K)
ノート
-
松原 守, 土屋 文彦2009 年 57 巻 4 号 p. 249-254
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーProtein phosphorylation is a key post-translational modification that governs biological processes. Methods for monitoring the phosphorylation status of proteins are very important for the evaluation of diverse biological and pathological processes. Here we analyzed the site-specific phosphorylation of myelin basic protein (MBP) by protein kinase JNK1 (c-Jun N-terminal protein kinase) using liquid chromatography tandem mass spectrometry and iTRAQ reagent. Samples from time course reactions were differentially tagged using a multiplex reagent system, which enabled the simultaneous identification and relative quantification of phosphorylation. Three phosphorylation sites (Thr94, Thr97, and Ser164) were identified; all of them contained the JNK1 consensus sequence. The observed time course patterns can be classified into three different types corresponding to phosphorylated peptides, unphosphorylated peptides, and peptides with no phosphorylation sites. The method enables a better understanding of the complexities of kinase activity through the efficient analysis of time-dependent effects.抄録全体を表示PDF形式でダウンロード (860K)
総合論文
-
南澤 俊和2009 年 57 巻 4 号 p. 255-281
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーI focus on the fragmentation rules derived by inspection of the multistage product ion mass spectra of glycosaminoglycan (GAG)-related carbohydrates. To establish a sophisticated sequencing method for GAGs using mass spectrometry, systematic understanding of fragmentation rules is essential. This review proposes an attractive protocol for GAG sequencing based on such knowledge, and shows that multiple-stage mass spectrometry is a powerful and promising technology to decipher highly heterogeneous GAG structures.抄録全体を表示PDF形式でダウンロード (3538K)
解説
-
伊藤 茂泰2009 年 57 巻 4 号 p. 283-289
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーNumerous studies have been reported on structural analysis of chondroitin sulfate (CS) oligosaccharides using multiple-stage mass spectrometry (MSn). However, their sequence determination is highly complex because of the simply repeating backbone structure. This study attempts to overcome this issue using an 18O-labeling method. The usefulness of the 18O-labeling method in the MSn analysis of CS oligosaccharide is shown in this paper.抄録全体を表示PDF形式でダウンロード (811K) -
—GLP (Good Laboratory Practice)フリーラボはバイオアナリシスで生き残れるか?—墳崎 靖子, 中野 英彦, 千田 直人2009 年 57 巻 4 号 p. 291-300
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーOur GLP (Good Laboratory Practice)-free laboratory continues to develop state-of-the-art technologies that will provide a cutting-edge service to our clients.
•We will respond to client requests promptly.
•We will have a good relationship with clients from the early stage of new drug development. Our GLP-free laboratory can supply many technologies for application to GLP laboratories.抄録全体を表示PDF形式でダウンロード (1495K) -
松田 知成, 永吉 晴奈, 椙村 春彦, 周 佩欣2009 年 57 巻 4 号 p. 301-304
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーFormation of DNA adducts is a crucial step for carcinogenesis and aging. However, until recently, it was difficult to quantify trace amount of DNA adducts in living organisms. Development of liquid chromatography/tandem mass spectrometry (LC/MS/MS) equipments enables us to quantify DNA adducts at practical sensitivity. Molecular epidemiological study such as aldehyde dehydrogenase 2 gene (ALDH2) genotypes and risk of alcohol-related DNA damage has been conducted by using LC/MS/MS. Furthermore, we developed “DNA adductome” analysis which can display comprehensive picture of DNA adducts in living organisms. These techniques may contribute to understand mechanisms of carcinogenesis and aging.抄録全体を表示PDF形式でダウンロード (464K) -
窪田 雅之2009 年 57 巻 4 号 p. 305-307
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーWe developed a new triple stage quadruple mass spectrometer equipped with a new ion guide based on “RF (radio frequency)-only stacked ring electrode technique.” This ion guide design improves ion transmission in differential pumping regions. This article introduces this design and its performance in comparison with traditional tube lens/skimmer lens designs.抄録全体を表示PDF形式でダウンロード (679K) -
—抗体中の残留フラグメント解析—千葉 かおり, 浮辺 雅宏, 陳 銀児, 志岐 成友, 大久保 雅隆2009 年 57 巻 4 号 p. 309-316
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーConventional time-of-flight mass spectrometers equipped with microchannel plate (MCP) detectors give only m/z values, which remains ambiguous over charge number (z). In contrast, the matrix-assisted laser desorption/ionization (MALDI) TOF mass spectrometer (TOF-MS) with a superconducting tunnel junction (STJ) detector—Super-TOF-MS—enables kinetic energy measurement with mass-independent detection efficiency. Therefore, since kinetic energy is proportional to z, it is possible to determine mass (m) unambiguously.
The Super-TOF-MS is effective for analysing high-mass fragments in immunoglobulin solution. Specifically, unknown peaks appearing on mass spectra for immunoglobulin G (IgG) samples can be identified as IgG fragments using kinetic energy measurements and the mass-independent detection efficiency of the STJ detector. A complete IgG molecule has two heavy and two light chains that are connected by disulfide bonds. The heavy and light chains include four and two structural domains, respectively (12 domains in total). The charge-state discrimination mass analysis enables the assignment of m values to the unknown peaks. The existence of pairs for which the m-value sum is equal to that of the intact IgG reveals that there are several fragment types consisting of different numbers of structural domain units.抄録全体を表示PDF形式でダウンロード (1379K) -
藤井 清永, 中野 智世, 碓井 史彦, 板東 泰彦, 稲垣 冬彦, 西村 俊秀2009 年 57 巻 4 号 p. 317-325
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーProtein identification via mass spectrometry (MS) relies primarily on protein sequence databases. Identification resulting from a sequence database search in the tandem mass spectrometry (MS/MS) experiments for the digested peptides is derived from an algorithm based on statistical confidence, and therefore, it often pro- ides false-positive identifications. Although one can apply high-resolution MS such as Fourier-transform mass spectrometer (FT-MS) to obtain results with high confidence, the instrumentation is expensive and the results are still probability based. Therefore, we introduced data-dependent triple stage mass spectrometry (MS3) into our well-established liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis of linear ion trap MS to achieve evidence-based protein identification. Peptide sequencing by MS3 analysis can provide a direct evidence for a sequence elucidated by MS/MS data and help determine whether the search results are correct.抄録全体を表示PDF形式でダウンロード (1272K)
Letter to the Editor
-
和田 芳直, 田中 耕一, 豊田 岐聡, 中村 健道2009 年 57 巻 4 号 p. 327-328
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーPDF形式でダウンロード (215K)
Message from the President
-
和田 芳直2009 年 57 巻 4 号 p. 329
発行日: 2009/08/01
公開日: 2009/08/15
ジャーナル フリーPDF形式でダウンロード (150K)
- |<
- <
- 1
- >
- >|