Flavonoids in edible part of onion were isolated and their structures were determined by SIMS and C-13 NMR comparing with the flavonol glucosides synthesized by Koenigs-Knorr reaction. The resultS were as follows: (1) By Koenigs-Knorr reaction using quercetin as an aglycon and tetraacetyl-1-α-bromoglucose, quercetin-3-O-β-glucoside, 4'-O-β-glucoside, 7-O-β-glucoside, 3, 4'-diO-β-glucoside, and 3, 7-diO-β-glucoside were obtained with the small amounts of 7, 4'-diO-β-glucoside. And their chemical structures, except 7, 4'-diO-β-glucoside, were determined by C-13 NMR. (2) By the same method using kaempferol as an aglycon, kaempferol-3-β-glucoside, 7-O-β-glucoside and 3, 7-diO-β-glucoside were obtained. And their structures were also determined by C-13 NMR. (3) The major flavonoid in onion is quercetin-4'-O-β-glucoside, closely followed by quercetin-3, 4'-diO-β-glucoside. And these two flavonols account for more than 85% of the total amount of flavonoids in onion. (4) The flavonoid account for more than 1% of total flavonoid in onion were quercetin-3-O-β-glucoside, 7-O-β-glucoside, 7, 4'-diO-β-glucoside and isorhamnetin-4'-O-β-glucoside. (5) The minor flavonoid, isorhamnetin-3, 4'-diO-β-glucoside, kaempferol-3, 4'-diO-β-glucoside and kaempferol-4'-O-β-glucoside were also detected in onion.
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