Effect of caprylohydroxamic acid (CHA), a potent urease inhibitor, on ammonia and urea levels, and urease activity in gastrointestinal contents of swine was studied.
Nine pigs were devided into two groups. Three pigs in treatment lot were basal diet containing 0.1%CHA and the others were fed the basal diet. Feed and water were supplied ad libitum througout the experiment.
The results were as follows;
1. In both control and treatment groups, ammonia concentration gradually increased from stomach to large intestine with changing the portions of gastrointestinal tract.
Compared with ammonia levels in both groups, the treatment pigs had a higher ammonia level than the control pigs at stomach and caecum. But inversely the latters showed a higher ammonia level than the former at jejunum, ileum and colon. However, there were no significant differences between control and treatment groups.
2. Urea concentration was increased at small intestine, but decreased at large intestine in both groups. Feeding of the diet containing CHA significantly increased urea concentration at duodenum and jejunum.
3. Urease activity was determined on sotmach, jejunum, colon and rectum contents.
The treatment pigs showed lower urease activity than the controls, but the statistical significance were only obtained at rectum.
4. These results suggest that urea hydyolysis is major source of exogenous ammonia in gastrointestinal contents and CHA inhibits the urease activity or the urea hydrolysis.
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