The authors carried out a series of experiments in winter and spring of 1974 on the treatment of waste water with a aeration tank (of a nominal capacity for 350 swine) made of FRP equipped with a mechanical aerator of the conventional activated sludge system located in Hikari-machi, Chiba Prefecture, Japan.
The results were as summarized hereunder.
(1) The conditions for the operation of the treatment in winter and spring comprised water temperature in the aeration tank of 14.7±0.2°C and 17.5±1.1°C, respectively, sludge volume at 30 minutes of 94.0±2.4% and 77.8±12.1%, sludge volume indice of 105.0±10.6 and 173.8±16.3, MLSS in the aeration tank of 9, 060±1, 010ppm and 4, 490±595ppm, and MLSS in the returned sludge of 20, 300±2, 330ppm and 7, 260±1, 210ppm, respectively.
(2) The detention time of waste water in the aeration tank were 12.5 hours and 11.5 hours respectively in winter and spring, while BOD loading in kg/m³ were 1.38±0.34kg/m³.d and 1.49±0.48kg/m³.d, in winter and spring, respectively. BOD kg loading per 1kg of MLSS in the aeration tank were 0.15±0.03kg/kg.d (winter) and 0.34±0.10kg/kg.d (spring).
(3) In the test on the functions of the vibration screen, the COD removal ratios determined were 33.5% and 27.9%, respectively in winter and spring at COD consistency of raw waste water of respectively 3, 910±1, 370ppm and 2, 380±1, 080ppm in winter and spring, respectively. SS removal ratios determined then were 23.4% and 28.8% at 7, 740±3, 360ppm and 7, 830±5, 120ppm in winter and spring, respectively.
(4) The capacity for the aeration tank to treat waste water comprised BOD consistency in efferent water of 97.1±16.0ppm in winter and 83.3±12.1ppm in spring and the BOD removal ratios were respectively 93.7% and 94.1%. The SS consistency in efferent was 38.8±13.9ppm in winter and 19.9±4.83ppm in spring, while the SS removal ratios were respectively 97.4% and 99.2%.
The nitrogen removal ratios determined comprised a total nitrogen of 68.8%, ammoniacal nitrogen of 49.2%, and albuminoid nitrogen of 81.8% in winter trial and 64.4%, 41.6%, and 82.5% in spring trial, respectively.
(5) The weights of excess in sludge formed from the aeration tank were 38.82kg/day in the winter trial and 36.17kg/day in the spring trial, which corresponded to 0.53kg/kg and 0.46kg/kg of removed BOD.
(6) BOD removal efficiency per ps. per hour amounted to 1.51kg. BOD/ps H. in the winter trial and 1.64kg BOD/ps. H. in the spring trial, which were about 3 times of that of the method with bubbling aeration of sludge.

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The authors earlier reported the results of the immunological studies on the source specificity of pork with the saline extracts and myoglobin from pork, beef, and horse meat.
1. The actomyosin antigen was prepared out of pork in accordance with the immunological method proposed by Knieriem.
2. Presence of two types of antigen-antibody systems were noted in the actomyosin antigen from pork in “the shape of the field of precipitation reaction” on the basis of the results of tests carried out in accordance with the stratifying method of the precipitation reaction.
3. The study with electrophoresis turned out that there were two peaks in the findings with the actomyosin antigen from pork.
4. Although presence of two types of antigen systems was noted with actomyosin from pork in accordance with the method of Ouchterlony, cross reactions were noted between the pork actomyosin and beef actomyosin, and between the pork actomyosin and horse meat actomyosin. It was, therefore, concluded that the actomyosin antigen would not be adequate as the antigen to be used for immunological identification of pork.

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The authors earlier reported the results of the immunological studies on the source specificity of pork with saline extracts, myoblobin, and actomyosin from pork, beef, and horse meat. The result showed that myoglobin was the most valuable antigen for identification of various types of meat.
In order to further study the applicability of this method to practical identification of various kinds of meat, hemagglutination test with erythrocytes treated with tannic acid was carried out in accordance with Boyden's method with myoglobin antigen from pork, the antigens from the extracts of pork, beef, and horse meat with physiological saline, gamma-globulins extracted from antisera to pork myoglobin, beef myoglobin, and myogloglobin of horse meat.
As a result, hemagglutination was detected, with specificity to species, between pork myoglobin antigen and gamma-globulin extracted from pork myoglobin antiserum, between the extract of pork with physiological saline and gamma-globulin extracted from antiserum of pork myoglobin, between the extract of beef with physiological saline and gamm-globulin extracted from antiserum of beef myoglobin, and between the extract of horse meat with physiological saline and gamma-globulin extracted from antiserum to myoglobin of horse meat, while no hemagglutination was noted between the extracts of these types of meat and gamma-globulins.
From the results as above, it was noted that identification of pork can be carried out with myoglobin using the hemagglutination reaction of erythrocytes treated with tannic acid.

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