The present study examined the morphological differences between the homozygous db+/db+ and the heterozygous db+/+m mice in pancreas and muscle tissue.
Method: Every mouse was six weeks old and female. Pancreas tissue was stained by the aldehyde-fuchsin method of Gomori. The area of Langerhans islet and the number of beta cells secreting the insulin were measured. Muscle tissue (gastrocnemius) was stained by the succinic dehydrogenase method (SDH method) . The numbers and area of red and white muscle fiber were measured.
Result: Neither did the area of Langerhans islet nor the number of beta cells in Langerhans islet has a significant difference. The numbers of beta cells in Langerhans islet (/mm
2) were significantly higher in the db+/+m mice than those of the db+/db+ mice (
p<0.05) . The ratio of area and the number in red muscle fibers were significant high in db+/db+ mice compared with db+/+m mice (
p<0.05) . There was no significant difference between mice respecting the size of red muscle fibers, however, the size of white muscle fiber was significantly larger in the db+/+m mice than that of the db+/db+ mice (
p<0.05) .
Discussion: In order to select animals that are genetically abnormal for control group, we should consider the morphological differences.
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