To confirm the previous results obtained from the field investigation on viral diseases of tomato plants in greenhouses, eight kinds of genetically different cultivars were mechanically inoculated with common strain of tobacco mosaic virus (TMV-OM), L strain of TMV (TMV-L), cucumber mosaic virus (CMV), and potato virus X (PVX) . The causal factors of necrotic symptom on these inoculated plants were analyzed on the basis of the relationship between the strains of TMV and TMV-resistant genes of tomato plants. Moreover, our attention was focused on the temperature which affects the appearance of symptoms.
With the inoculation of TMV-OM and TMV-L, mosaic symptom accompanied with fern leaf was observed on Fukuju-2 and Ponterosa, susceptible plants. FTVR-50, heterozygous plant of Tm-1 gene, and GCR-237 (homozygous one) were tolerant to both TMV strains. Reiko, heterozygous plant of Tm-1, Tm-2
nv and Tm-2
a genes, and GCR-267, homozygous one of Tm-2
a, were immune to both TMV strains. On the other hand, both TMV strains caused typical necrotic symptom on Ogatazuiko, heterozygous plant of Tm-2
a gene. In the case of GCR-236, homozygous plant of Tm-2
nv, necrotic symptom was observed in 3 out of 8 plants by the inoculation of TMV-L although this cultivar wad immune to TMV-OM. CMV caused fern leaf or mosaic symptom accompanied with fern leaf, and PVX caused mosaic symptom with ringspot in eight cultivars used.
The tomato strain of TMV (TMV-T) isolated from diseased tomato plants with mosaic or necrotic symptom in Shizuoka Prefecture also caused necrotic symptom on Ogatazuiko. Since Reiko was immune to TMV-T isolated from diseased Reiko grafted on BF-101, the necrosis observed on Reiko in the greenhouses seems to be caused by physiological changes in grafting but not by the virus changed in pathogenicity.
Ogatazuiko inoculated with TMV-OM or TMV-L was incubated at 17, 22, and 27°C to confirm whether or not the appearance of symptom on tomato plant having Tm-2
a gene depends on temperature. The necrotic symptom appeared at a temperature higher than 22°C by the inoculation of TMV-OM, but the necrotic symptom appeared at a temperature higher than 17°C by the inoculation of TMV-L.
In control of viral necrotic disease of tomato plants in greenhouses, particular attention must be paid to the selection of immune or tolerant cultivars to the dominantly distributed TMV strain and regulation of temperature.
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