Orchids of
Brassolaeliocattleya Chian-Tzy Emperor (
Cattleya) and
Cymbidium Sazanami were cultured at 32/20°C, 30/15°C, and 28/8°C (maximum/minimum temperature) for 2 months. It was found in
Cattleya leaves that superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) activities were lower than in
Cymbidium leaves, while polyphenol oxidase (PPO) and ascorbate oxidase (AOX) activities were higher. The high cultural temperature enhanced activities in all enzymes in
Cattleya and in four enzymes except CAT in
Cymbidium, indicating that it greatly affects scavenging enzyme activities compared to the low temperatures which induced only an increase in APX activity in
Cattleya and a decrease in CAT activity in
Cymbidium. PPO activity increased and AOX decreased in both orchids at the low temperature.
Cattleya leaves at both high and low cultural temperatures decreased ascorbic acid, while
Cymbidium increased it.
Cattleya at the low temperature increased markedly SH-containig substances but the latter at the high temperature decreased these substances. Lipid peroxides increased in
Cymbidium leaves under both high and low temperatures, but for
Cattleya neither temperature conditions affected lipid peroxides.
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