Changes in the distribution of low molecular weight carbohydrates, polysaccharides, and organic acids in mycelia during the vegetative growth of Lyophyllum shimeji and Lyophyllum fumosum were studied. On a dry-weight basis the low molecular weight carbohydrate content during vegetative growth was in the range of 2.9-9.8% in L. shimeji and 1.9-10.0% in L. fumosum. Trehalose, glucose, fructose, mannitol, arabitol and glycerol were identified in the mycelia, of which trehalose was the major component. On the same basis, the polysaccharide content was in the range of 54.0-62.4% in L. shimeji and 48.4-62.9% in L. fumosum. The acetic acid-soluble (glycogen) fraction was predominant in the polysaccharide fractions in the vegetative mycelia of both species. Trehalose and glycogen accumulated in the vegetative mycelia were thought to serve as carbohydrate reserves. The organic acid content was in the range of 1.3-1.6% in L. shimeji and 1.8-2.2% in L. fumosum, and eleven organic acids were identified. Malic, citric, pyroglutamic and fumaric acids were predominant in L. shimeji, and lactic, malic, citric, pyroglutamic and fumaric acids were predominant in L. fumosum. Lactic acid was found to accumulate in the mycelium of L. fumosum during vegetative growth.

　Two species of yeast were isolated from wood vinegar and identified. One species, isolated from wood vinegar extracted from Japanese oaks, was Dipodascus capitatus. The other species, isolated from wood vinegar extracted from North American maples, was Rhodotorula minuta.